Study on the relationship between the expression of ETFA gene and clinicopathological parameters and prognosis in breast cancer

doi:10.3877/cma.j.issn.1674-3946.2025.01.018.

Abstract

Abstract:

Objective

To investigate the relationship between the expression of electron transfer flavin protein A （ETFA） and Hematopoietic progenitor cell antigen （CD34） and clinicopathological parameters and prognosis in breast cancer.

Methods

A total of 115 female breast cancer patients who underwent surgical treatment from December 2020 to February 2024 were retrospectively collected and followed up for 36 months.Statistical software SPSS 22.0 was used to analyze the data.The measurement data conforming to the normal distribution were represented by （x ± s）， and the independent sample t was compared.The counting data were represented by［ cases （%）］ and compared by χ² test.The positive expression of ETFA and CD34 in breast cancer tissues was correlated with different pathological parameters by Spearson analysis.Kaplan-Meier survival curve was used to analyze the 3-year survival rate of breast cancer patients.P＜0.05 indicated that the difference was statistically significant.

Result

There were significant differences in the expression of ETFA and CD34mRNA in adenocarcinoma tissues and adjacent tissues （P＜0.05）.The positive expression rates of ETFA and CD34 in breast cancer tissues were 73.9% and 77.4%， respectively， and the expression rates in adjacent tissues were 25.2% and 9.6%， respectively， with statistical significance （P＜0.05）.The positive expression rates of ETFA and CD34 in breast cancer tissues were significantly different in tumor stage， differentiation degree and lymph node metastasis （P＜0.05）.Spearson correlation analysis showed that the positive expression of ETFA and CD34 in breast cancer tissues was positively correlated with tumor stage and lymph node metastasis， and negatively correlated with tumor differentiation.The 3-year survival rate of ETFA-positive breast cancer patients was 55.3%， lower than that of ETFA-negative breast cancer patients （59.0%， P=0.021）.The 3-year survival rate for patients with CD34-positive breast cancer was 51.6%， compared with 62.7% for patients with CD34-negative breast cancer （P=0.000）.

Conclusion

The high expression of ETFA mRNA and CD34mRNA in breast cancer tissues is closely related to the development of tumor deterioration and poor prognosis of patients，with a view to becoming a marker to evaluate the severity of breast cancer patients.

Key words: Breast Neoplasms, Electron Transfer Flavprotein A, Prognosis

Jiehong Gao, Pingping Li, Jing Qi, Yinhai Dai. Study on the relationship between the expression of ETFA gene and clinicopathological parameters and prognosis in breast cancer[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 64-67.

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References 15

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临床病理参数	例数	ETFA阳性（n=85）	P值	CD34阳性（n=89）	P值
年龄（岁）
＜50	63	49	0.299	46	0.217
≥50	52	36	0.299	43	0.217
肿瘤直径（cm）
＜4cm	45	30	0.156	37	0.321
≥4	70	55	0.156	52	0.321
肿瘤分期
Ⅰ-Ⅱ期	58	35	0.000	40	0.029
Ⅲ-Ⅳ期	57	50	0.000	49	0.029
肿瘤分化程度
高分化	25	19	0.024	18	0.023
中分化	36	31		27
低分化	54	35		44
淋巴结转移
是	72	45	0.000	49	0.001
否	43	40	0.000	40	0.001

临床病理参数	例数	ETFA阳性（n=85）	P值	CD34阳性（n=89）	P值
年龄（岁）
＜50	63	49	0.299	46	0.217
≥50	52	36	0.299	43	0.217
肿瘤直径（cm）
＜4cm	45	30	0.156	37	0.321
≥4	70	55	0.156	52	0.321
肿瘤分期
Ⅰ-Ⅱ期	58	35	0.000	40	0.029
Ⅲ-Ⅳ期	57	50	0.000	49	0.029
肿瘤分化程度
高分化	25	19	0.024	18	0.023
中分化	36	31		27
低分化	54	35		44
淋巴结转移
是	72	45	0.000	49	0.001
否	43	40	0.000	40	0.001

指标	肿瘤分期		淋巴结转移		肿瘤分化程度
指标	r	P值	r	P值	r	P值
ETFA	0.658	0.023	0.716	0.018	-0.589	0.022
CD34	0.677	0.041	0.697	0.044	-0.641	0.007

指标	肿瘤分期		淋巴结转移		肿瘤分化程度
指标	r	P值	r	P值	r	P值
ETFA	0.658	0.023	0.716	0.018	-0.589	0.022
CD34	0.677	0.041	0.697	0.044	-0.641	0.007

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