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中华普外科手术学杂志(电子版)

中华普外科手术学杂志(电子版) ›› 2018, Vol. 12 ›› Issue (06) : 468 -471. doi: 10.3877/cma.j.issn.1674-3946.2018.06.007

所属专题： 文献；

论著

甲状腺乳头状癌组织TSHR表达与BRAFV600E基因突变的关系研究
郝少龙1, 马纪红2, 刘新承3, 陈焕杰3, 郑海涛3,†(), 宋西成4,†()   
  1. 1. 101149　首都医科大学附属北京潞河医院普外科
    2. 101149　首都医科大学附属北京潞河医院通科内科
    3. 264000　青岛大学医学院附属烟台毓璜顶医院甲状腺外科
    4. 青岛大学医学院附属烟台毓璜顶医院耳鼻喉头颈外科
  • 收稿日期:2017-06-26 出版日期:2018-12-26
  • 通信作者: 郑海涛, 宋西成

Clinical investigation of the expression of TSHR and BRAFV600E gene mutation in papillary thyroid carcinoma

Shaolong Hao1, Jihong Ma2, Xincheng Liu3, Huanjie Chen3, Haitao Zheng3,†(), Xicheng Song4,†()   

  1. 1. Department of General Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing
    2. Department of General medicine, Beijing Luhe Hospital, Capital Medical University, Beijing
    3. Department of Thyroid Surgery, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Shandong 264000, China
    4. Department of Otorhinolaryngology-Head & Neck Surgery, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Shandong 264000, China
  • Received:2017-06-26 Published:2018-12-26
  • Corresponding author: Haitao Zheng, Xicheng Song
  • About author:
    Corresponding author: Zheng Haitao, Email:
    Song Xicheng, Email:
  • Supported by:
    Natural Science Fundation of Shandong Province(ZR2015HL092); Key Research Project of Shandong Province(2015GSF118090); Municipal Scientific and Technological Development Project of Yantai city(2015YT06000060)
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郝少龙, 马纪红, 刘新承, 陈焕杰, 郑海涛, 宋西成. 甲状腺乳头状癌组织TSHR表达与BRAFV600E基因突变的关系研究[J]. 中华普外科手术学杂志(电子版), 2018, 12(06): 468-471.

Shaolong Hao, Jihong Ma, Xincheng Liu, Huanjie Chen, Haitao Zheng, Xicheng Song. Clinical investigation of the expression of TSHR and BRAFV600E gene mutation in papillary thyroid carcinoma[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2018, 12(06): 468-471.

目的

探究甲状腺乳头癌组织中促甲状腺激素受体(TSHR)及TSHR－mRNA的表达与BRAFV600E基因突变的关系。

方法

收集甲状腺乳头状癌患者组织及正常组织标本122例，利用PCR法检测甲状腺癌组织中BRAFV600E基因突变情况，并将其分为BRAFV600E基因突变组和BRAFV600E基因野生组；应用免疫组织化学法检测甲状腺乳头状癌组织中TSHR的表达情况，并应用Western blot和RT－PCR法进行验证。应用SPSS17.0统计学软件对数据进行分析。采用χ2检验和独立样本t检验分析TSHR及TSHR－mRNA的表达与BRAFV600E基因突变的关系，P<0.05认为差异有统计学意义。

结果

TSHR及TSHR－mRNA在甲状腺乳头状癌组织中表达量明显降低(P<0.05)。BRAFV600E基因突变组的甲状腺乳头状癌组织中TSHR及TSHR－mRNA表达水平较BRAFV600E基因野生型组明显降低。

结论

甲状腺乳头状癌组织中BRAFV600E基因突变与TSHR表达下调有关。

关键词: 甲状腺肿瘤, 癌，乳头状, 受体，促甲状腺素
Objective

To explore the relationship between expression of TSHR, TSHR-mRNA and BRAFV600E gene mutation in papillary thyroid cancer.

Method

A total of 122 patients with papillary thyroid cancer were enrolled in this study. PCR was applied to detect gene mutation of BRAFV600E in papillary thyroid cancers. All the patients were divided into BRAFV600E gene mutation group and BRAFV600E gene wild-type group. Immunohistochemical assay were used to detect expression of TSHR in papillary thyroid cancers. Western blotting and Real-time quantitative PCR (RT-PCR) were used to verify the results. Statistical analysis was performed by using SPSS17.0 software. Chi-squared test and Independent sample t test were applied to analyze the relationship between the expressions of TSHR, TSHR-mRNA and BRAFV600E gene mutation. A P value <0.05 was considered as statistical significance.

Result

The expression levels of TSHR and TSHR-mRNA in papillary thyroid cancers was significant decreased (P<0.05), and the expression of TSHR and TSHR-mRNA in papillary thyroid cancers were decreased more obviously in BRAFV600E gene mutation group than those in BRAFV600E gene wild-type group.

Conclusion

BRAFV600E gene mutation is associated with the lower expression of TSHR in papillary thyroid cancers.

Key words: Thyroid Neoplasms, Carcinoma, Papillary, Receptors, Thyrotropin
表1 122例PTC组织中BRAF基因突变组和野生组TSHR表达分析(例)
基因型 阴性 弱阳性 阳性 强阳性 合计
BRAF基因突变型 2 26 36 8 72
BRAF基因野生型 1 10 24 15 50
合计 3 36 60 23 122
基因型 χ2值 df P值 95% CI
BRAF基因突变型 8.277 3 0.032 0.027～0.036
BRAF基因野生型
图1 PTC组织中TSHR同时阳性表达于细胞膜和细胞质中(HE×400)
图2 TSHR在PTC组织中的表达情况(免疫组织化学SP法，HE×400)[A.阴性表达　B．弱阳性表达　C．阳性表达　D．强阳性表达]
图3 PTC组织中TSHR的表达量Western blot试验结果　正常甲状腺对照组织(1.2.3)、BRAF基因野生型PTC组织(4.5.6)及BRAF基因突变型PTC组织(7.8.9)TSHR的表达情况。
图4 BRAF基因突变型及野生型PTC组织与正常对照甲状腺组织TSHRmRNA的相对表达量(RT－PCR)
图6 BRAFV600E基因突变型和BRAFV600E基因野生型PTC组织TSHRmRNA RT－PCR试验结果
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