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中华普外科手术学杂志(电子版)

中华普外科手术学杂志(电子版) ›› 2018, Vol. 12 ›› Issue (03) : 246 -249. doi: 10.3877/cma.j.issn.1674-3946.2018.03.020

所属专题： 文献；

论著

回直肠侧侧吻合分流术对慢传输型便秘大鼠血清和结肠组织匀浆中5－HT含量的影响
张卫平1, 吴银亚2, 江滨3,†(), 丁曙晴3, 皇甫少华3   
  1. 1. 214100　解放军101医院（无锡太湖医院）普通外科
    2. 214100　解放军101医院（无锡太湖医院）消化内科
    3. 210000　南京市中医院全国中医肛肠医疗中心
  • 收稿日期:2018-05-25 出版日期:2018-06-26
  • 通信作者: 江滨

Effects of the ileo-rectal anastomosis bypass procedure on 5-HT in the blood plasma of the rats with slow transit constipation

Weiping Zhang1, Yinya Wu2, Bin Jiang3,†(), Shuqing Ding3, shaohua HuangFu3   

  1. 1. Department of General Surgery, the 101 Hospital of PLA, Wuxi 214000, China
    2. Department of Digestion, the 101 Hospital of PLA, Wuxi 214000, China
    3. National Medical Centre of Colorectal Disease, Nanjing Hospital of Traditon Chinese Medcine, Nanjing 210000, Jiangsu Province, China
  • Received:2018-05-25 Published:2018-06-26
  • Corresponding author: Bin Jiang
  • About author:
    Corresponding author: Jiang Bin, Email:
  • Supported by:
    The Key Fund Project of Nanjing Municipal Health Bureau(No. ZKX11004); The Scientific and Technologic Development Programme of Nanjing(No. 201108004); The Fund Project of Chinese Medicine in Jiangsu Province(No. LZ11105)
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张卫平, 吴银亚, 江滨, 丁曙晴, 皇甫少华. 回直肠侧侧吻合分流术对慢传输型便秘大鼠血清和结肠组织匀浆中5－HT含量的影响[J]. 中华普外科手术学杂志(电子版), 2018, 12(03): 246-249.

Weiping Zhang, Yinya Wu, Bin Jiang, Shuqing Ding, shaohua HuangFu. Effects of the ileo-rectal anastomosis bypass procedure on 5-HT in the blood plasma of the rats with slow transit constipation[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2018, 12(03): 246-249.

目的

完成慢传输型便秘(STC)大鼠回肠直肠吻合分流手术，观察该术后STC大鼠血清、结肠中五羟色胺(5－HT)值的变化。

方法

72只SD大鼠，造模成功后(造模过程中死亡5只)完成回肠直肠吻合分流术，最终对照组10只，模型组12只，恢复组10只，手术组35只(术中死亡3只)；测定并比较各组大鼠的血清、结肠中5－HT的含量。采用SPSS17.0软件进行数据分析。所有的统计检验均采用双侧检验，大鼠血清、结肠组织中5－HT值的变化采用±s表示，组间比较采用两独立样本的t检验，P<0.05为差异有统计学意义。

结果

血清中5－HT：与对照组相比，模型组大鼠血清5－HT水平显著增高(P＝0.002<0.01)；恢复组与模型组对比无明显差异(P＝0.351>0.05)，但明显高于对照组(P＝0.001<0.01)。术后1个月，手术组与恢复组差异无统计学意义(P＝0.171>0.05)。结肠组织匀浆中5－HT：与正常对照组相比，模型组、恢复组、手术组大鼠结肠组织匀浆中5－HT均显著升高(P<0.01)；恢复组与模型组差异无统计学意义(P＝0.263>0.05)，手术后大鼠结肠组织中5－HT较恢复组增高。

结论

STC大鼠血清及结肠组织中5－HT值明显增高，回直肠吻合分流术能改善STC大鼠的排便情况，但术后结肠中5－HT仍增高，结肠功能未能恢复。

关键词: 便秘, 回肠, 直肠, 吻合术，外科, 血清素
Objective

To investigate the influence of the ileo-rectal anastomosis bypass procedure on on the 5-HT in the blood plasma of therats with STC.

Methods

72 SD rats were completed after the model was successful (5 dead in the process of modeling) and completed the ileo rectal anastomosis operation. The final control group was 10, 12 in the model group, 10 in the recovery group and 35 in the operation group (3 in the operation), and the content of 5-HT in the serum and colon of the rats was measured and compared. Statistical analyses were performed using SPSS17.0 software. Two-sided test was used in all statistical tests. The value of 5-HT in the serum and colon were presented by ±s. Comparisons between two groups were made by independent t test. Only differences of P<0.05 were considered to be significant.

Result

5-HT in serum: Compared with normal control group, 5-HT in model group was increased considerably (P=0.002<0.01). There was no significant difference between operation group and recovery group (P=0.171>0.05), but operation group was still higher than model group. 5-HT in colon: Compared with normal control group, 5-HT was significant increased in model group, recovery group, and operation group (P<0.01); While there was no significant deviation between control group and recovery group. Operation group was higher than recovery group (P=0.001<0.05).

Conclusion

5-HT was significantly increased in the rats of STC model constipation. The ileo-rectal anastomosis bypass procedure do a great improvement in symptom of STC rats, but 5-HT in rats after surgery is still increased, The role of 5-HT in gastrointestinal motility should be further studied.

Key words: Constipation, Ileum, Rectum, Anastomosis, Surgical, Serotonin
图2 缝合吻合口
图4 部分大鼠出现结肠肠管胀气扩张
图6 吻合口通畅
表1 不同组别的64只STC大鼠血清及结肠组织中5－HT检测结果(±s)
组别 只 血清5－ht含量(ng/ml) 结肠5－HT含量(ng/mg)
对照组 10 5.44±1.04 9.94±1.17
模型组 12 8.45±1.54 14.79±1.52
恢复组 10 8.12±1.78 13.94±1.98
手术组 32 8.71±1.95 16.48±2.08
多重比较：P值 ? ? ?
? 对照组vs模型组 ? 0.002 0.000
? 模型组vs恢复组 ? 0.351 0.263
? 恢复组vs手术组 ? 0.171 0.001
? 对照组vs手术组 ? 0.001 0.000
[1]
El－Salhy M, Svensen R, Hatlebakk JG, et al. Chronic constipation and treatment options (Review)[J]. Mol Med Rep，2014,9(1):3-8.
[2]
黄忠诚．慢传输型便秘手术的规范与实施[J]．中华胃肠外科杂志，2016,19(12):1338-1341.
[3]
江滨，丁曙晴，丁义江，等．腹腔镜回肠直肠侧侧吻合分流术治疗顽固性结肠慢传输型便秘的临床研究[J]．临床外科杂志，2010,18(12):822-825.
[4]
张卫平，江滨，丁曙晴，等．回直肠吻合分流术对慢传输型便秘大鼠排便影响的初步研究[J]．世界华人消化杂志，2012,20(6):509-213.
[5]
俞媛洁，陈继红.5－羟色胺与胃肠动力障碍的关系[J]．医学综述，2016, 22(4):636-639.
[6]
Cao H, Liu X, An Y, et al. Dysbiosis contributes to chronic constipation development via regulation of serotonin transporter in the intestine[J]．Scientific Reports , 2017 , 7(1):10322.
[7]
Kon R, Ikarashi N, Hayakawa A, et al. Morphine－Induced Constipation Develops With Increased Aquaporin－3 Expression in the Colon via Increased Serotonin Secretion[J]. Toxicol Sci，2015,145(2):337-347.
[8]
李迪夫，刘铜军. 慢传输型便秘外科治疗的主要术式及疗效[J/CD]. 中华结直肠疾病杂志(电子版), 2016, 5(1):68-72.
[9]
Mawe GM, Hoffman JM.Serotonin signalling in the gut－－functions, dysfunctions and therapeutic targets[J]．Nat Rev Gastroenterol Hepatol, 2013,10(8):473-486.
[10]
赵兵，孔鹏飞，吴至久，等. 5－HT与慢传输型便秘相关性的研究[J/CD]. 中华结直肠疾病杂志(电子版), 2016, 5(6):499-502.
[11]
夏利锋，罗菊芳，冯跃，等．腹腔镜下回肠直肠侧侧吻合术治疗顽固性结肠慢传输型便秘的效果[J]. 广东医学，2015,36(5):748-749.
[12]
Omer A, Quigley EMM. An update on prucalopride in the treatment of chronic constipation[J]. Therap Adv Gastroenterol，2017,10(11):877-887.
[13]
Yu Y, Chen JH, Li H, et al. Involvement of 5－HT3 and 5－HT4 receptors in colonic motor patterns in rats[J]. Neurogastroenterol Motil，2015 ,27(7):914-928.
[14]
Kendig DM, Grider JR.Serotonin and Colonic Motility[J]．Neurogastroenterol Motil，2015,27(7):899-905.
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