Study on the relationship between the expression of VEGFR2 and miR-27a-5p in gastric cancer tissues and clinicopathological parameters and prognosis

doi:10.3877/cma.j.issn.1674-3946.2024.05.024

Abstract

Abstract:

Objective

To study the relationship between the expression of VEGFR2 and miR-27a-5p in gastric cancer tissues, clinicopathological parameters and prognosis.

Methods

Data of 98 patients with gastric cancer who underwent laparoscopic total gastrectomy from January 2020 to December 2022 were retrospectively analyzed. Immunohistochemistry (IHC) and real-time quantitative fluorescent PCR (qRT-PCR) were used to detect the expression of VEGFR2 and miR-27a-5p mRNA in gastric cancer tissues and corresponding paracancer tissues (>5cm from the margin of cancer tissues). SPSS 20.0 software was used for statistical analysis. The expression level of miR-27a-5p and other quantitative data were expressed by () and independent sample t-test was performed. Clinical and pathological parameters and other statistical data were represented by [cases (%)], row Rank Sum test or χ² test. Spearman Rank correlation was used to analyze the correlation between VEGFR2 and miR-27a-5p expression in gastric cancer tissues. The relationship with prognosis was analyzed by Kaplan-Meier method and Log-Rank test. P<0.05 indicates statistical difference.

Results

The high expression rate of VEGFR2 in gastric cancer tissues was significantly higher than that in para-cancer tissues, and the expression level of miR-27a-5p in gastric cancer tissues (1.08±0.42) was significantly lower than that in para-cancer tissues (2.51±0.85), with statistical significance (P<0.05), and the expression of the two was negatively correlated (r=-0.462, P=0.003). The high expression of VEGFR2 was significantly correlated with lesion diameter, T stage, differentiation degree, pTMN stage, vascular invasion, and lymph node metastasis (P<0.05), while the low expression of miR-27a-5p was significantly correlated with tumor differentiation degree, pTMN stage, vascular invasion, and lymph node metastasis (P<0.05). Kaplan-Meier analysis showed that the cumulative overall survival rate of the VEGFR2 high expression group was significantly lower than that of the VEGFR2 low expression group (81.0% vs. 94.7%) (P=0.047). The cumulative overall survival rate of miR-27a-5p expression group was significantly lower than that of miR-27a-5p expression group (81.7% vs. 92.6%) (P=0.041).

Conclusion

VEGFR2 is highly expressed in gastric cancer tissues, while miR-27a-5p is low expressed in gastric cancer tissues, and there may be interaction between the two, which is involved in the growth, proliferation, differentiation, invasion and migration of gastric cancer, thus affecting the course of gastric cancer, and is expected to become a potential target for diagnosis, treatment and prognosis evaluation of gastric cancer.

Key words: Stomach Neoplasms, Vascular Endothelial Growth Factor Receptor-2, Cell Proliferation, Clinicopathological Parameters, Prognosis

Fu Huang, Qian Wang, Xiangren Jin, Yunchuan Tang. Study on the relationship between the expression of VEGFR2 and miR-27a-5p in gastric cancer tissues and clinicopathological parameters and prognosis[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(05): 558-561.

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URL: https://zhpwkssxzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-3946.2024.05.024

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References 13

［1］	Li H，Zhang H，Zhang H，et al. Survival of gastric cancer in China from 2000 to 2022: A nationwide systematic review of hospital-based studies［J］. J Glob Health，2022,12:11014.
［2］	崔伟，李涛，李世拥. 中国腹腔镜胃癌手术20年历程与成就［J/CD］. 中华普外科手术学杂志（电子版），2021,15(02):139-142.
［3］	Ahmad A，Nawaz MI. Molecular mechanism of VEGF and its role in pathological angiogenesis［J］. J Cell Biochem，2022,123(12):1938-1965.
［4］	Kasai S，Kuwayama N，Motoo Y，et al. Dual blockade of MET and VEGFR2 signaling pathways as a potential therapeutic maneuver for peritoneal carcinomatosis in scirrhous gastric cancer［J］. Biochem Biophys Res Commun，2022,600:80-86.
［5］	赵蓓蓓，吴帅. miR-27a，miR-138-5p对食管癌的早期诊断价值及与其病理分化程度的关系研究［J］. 实用癌症杂志，2023,38(05):722-725,729.
［6］	Chen W，Tan X，Yang Q，et al. MALAT1 enhances gemcitabine resistance in non-small cell lung cancer cells by directly affecting miR-27a-5p/PBOV1 axis［J］. Cell Signal，2022,94:110326.
［7］	Che X，Jian F，Chen C，et al. PCOS serum-derived exosomal miR-27a-5p stimulates endometrial cancer cells migration and invasion［J］. J Mol Endocrinol，2020,64(1):1-12.
［8］	Wambecke A，Ahmad M，Morice PM，et al. The lncRNA 'UCA1' modulates the response to chemotherapy of ovarian cancer through direct binding to miR-27a-5p and control of UBE2N levels［J］. Mol Oncol，2021,15(12):3659-3678.
［9］	He H，Song F，Gao Q，et al. The APEX1/miRNA-27a-5p axis plays key roles in progression，metastasis and targeted chemotherapy of gastric cancer［J］. Int J Pharm，2021,599:120446.
［10］	张伟，蔡振花. 早期胃癌患者腹腔镜胃癌根治术后复发相关因素分析［J/CD］. 中华普外科手术学杂志（电子版），2020,14(06):566-568.
［11］	Di Staso F，Di Pippo M，Abdolrahimzadeh S. Choroidal Neovascular Membranes in Retinal and Choroidal Tumors: Origins，Mechanisms，and Effects［J］. Int J Mol Sci，2023,24(2):1064.
［12］	Eguchi R，Kawabe JI，Wakabayashi I. VEGF-Independent Angiogenic Factors: Beyond VEGF/VEGFR2 Signaling［J］. J Vasc Res，2022,59(2):78-89.
［13］	Cui Y，Pu R，Ye J，et al. LncRNA FAM230B Promotes Gastric Cancer Growth and Metastasis by Regulating the miR-27a-5p/TOP2A Axis［J］. Dig Dis Sci，2021,66(8):2637-2650.

临床资料	类别	例（%）	临床资料	类别	例（%）
性别	男	58(59.2)	肿瘤直径（cm）	<5 cm	56(57.1)
性别	女	40(40.8)	肿瘤直径（cm）	≥5 cm	42(42.9)
年龄（岁）	<60	45(45.9)	pTMN分期	Ⅰ	33(33.7)
年龄（岁）	≥60	53(54.1)		Ⅱ	44(44.9)
BMI（kg/m²）	<23.5	60(61.2)		Ⅲ	21(21.4)
BMI（kg/m²）	≥23.5	38(38.8)	淋巴结转移	有	54(55.1)
T分期	T₁	17(17.3)	淋巴结转移	无	44(44.9)
	T₂	38(38.8)	神经侵犯	有	47(48.0)
	T₃	43(43.9)	神经侵犯	无	51(52.0)
脉管侵犯	有	60(61.2)	HER-2	阳性	45(45.9)
脉管侵犯	无	38(38.8)	HER-2	阴性	53(54.1)
分化程度	高	35(35.7)	Ki-67	高表达	38(38.8)
	中	45(46.7)	Ki-67	低表达	60(61.2)
	低	18(18.4)

临床资料	类别	例（%）	临床资料	类别	例（%）
性别	男	58(59.2)	肿瘤直径（cm）	<5 cm	56(57.1)
性别	女	40(40.8)	肿瘤直径（cm）	≥5 cm	42(42.9)
年龄（岁）	<60	45(45.9)	pTMN分期	Ⅰ	33(33.7)
年龄（岁）	≥60	53(54.1)		Ⅱ	44(44.9)
BMI（kg/m²）	<23.5	60(61.2)		Ⅲ	21(21.4)
BMI（kg/m²）	≥23.5	38(38.8)	淋巴结转移	有	54(55.1)
T分期	T₁	17(17.3)	淋巴结转移	无	44(44.9)
	T₂	38(38.8)	神经侵犯	有	47(48.0)
	T₃	43(43.9)	神经侵犯	无	51(52.0)
脉管侵犯	有	60(61.2)	HER-2	阳性	45(45.9)
脉管侵犯	无	38(38.8)	HER-2	阴性	53(54.1)
分化程度	高	35(35.7)	Ki-67	高表达	38(38.8)
	中	45(46.7)	Ki-67	低表达	60(61.2)
	低	18(18.4)

病理特征		例数	VEGFR2表达[例（%）]	P值	miR-27a-5p表达（）	P值
性别	男	58	46(79.3)	0.695	1.14±0.35	0.126
性别	女	40	33(82.5)	0.695	1.06±0.32	0.126
年龄（岁）	<60	45	35(77.8)	0.513	1.12±0.36	0.081
年龄（岁）	≥60	53	44(83.0)	0.513	1.02±0.34	0.081
BMI（kg/m²）	<23.5	60	48(80.0)	0.847	1.05±0.33	0.134
BMI（kg/m²）	≥23.5	38	31(81.6)	0.847	1.13±0.37	0.134
病灶直径（cm）	<5 cm	56	32(57.1)	0.035	1.14±0.40	0.054
病灶直径（cm）	≥5 cm	42	37(88.1)	0.035	1.01±0.38	0.054
T分期	T₁	12	6(50.0)	0.002	1.32±0.35	0.600
	T₂	33	25(75.8)		1.24±0.33
	T₃	38	34(89.5)		0.86±0.24
	T₄	15	14(93.3)		0.81±0.21
分化程度	高	35	24(68.6)	0.009	1.50±0.25	0.046
	中	45	40(88.9)		1.28±0.23
	低	18	15(83.3)		0.69±0.21
pTMN分期	Ⅰ	33	23(69.7)	0.004	1.56±0.31	0.047
	Ⅱ	44	38(86.4)		1.48±0.28
	Ⅲ	21	18(85.7)		0.74±0.22
脉管侵犯	有	60	53(83.3)	0.015	1.02±0.30	0.044
脉管侵犯	无	38	26(68.4)	0.015	1.13±0.32	0.044
神经侵犯	有	47	41(87.2)	0.111	1.06±0.34	0.284
神经侵犯	无	51	38(74.5)	0.111	1.10±0.35	0.284
淋巴结转移	有	54	50(92.6)	0.001	0.98±0.26	0.018
淋巴结转移	无	44	29(65.9)	0.001	1.12±0.39	0.018
HER-2	阳性	45	33(73.3)	0.093	0.99±0.22	0.074
HER-2	阴性	53	46(86.8)	0.093	1.06±0.25	0.074
Ki-67	高表达	38	28(73.7)	0.167	0.96±0.31	0.086
Ki-67	低表达	60	51(85.0)	0.167	1.05±0.32	0.086

病理特征		例数	VEGFR2表达[例（%）]	P值	miR-27a-5p表达（）	P值
性别	男	58	46(79.3)	0.695	1.14±0.35	0.126
性别	女	40	33(82.5)	0.695	1.06±0.32	0.126
年龄（岁）	<60	45	35(77.8)	0.513	1.12±0.36	0.081
年龄（岁）	≥60	53	44(83.0)	0.513	1.02±0.34	0.081
BMI（kg/m²）	<23.5	60	48(80.0)	0.847	1.05±0.33	0.134
BMI（kg/m²）	≥23.5	38	31(81.6)	0.847	1.13±0.37	0.134
病灶直径（cm）	<5 cm	56	32(57.1)	0.035	1.14±0.40	0.054
病灶直径（cm）	≥5 cm	42	37(88.1)	0.035	1.01±0.38	0.054
T分期	T₁	12	6(50.0)	0.002	1.32±0.35	0.600
	T₂	33	25(75.8)		1.24±0.33
	T₃	38	34(89.5)		0.86±0.24
	T₄	15	14(93.3)		0.81±0.21
分化程度	高	35	24(68.6)	0.009	1.50±0.25	0.046
	中	45	40(88.9)		1.28±0.23
	低	18	15(83.3)		0.69±0.21
pTMN分期	Ⅰ	33	23(69.7)	0.004	1.56±0.31	0.047
	Ⅱ	44	38(86.4)		1.48±0.28
	Ⅲ	21	18(85.7)		0.74±0.22
脉管侵犯	有	60	53(83.3)	0.015	1.02±0.30	0.044
脉管侵犯	无	38	26(68.4)	0.015	1.13±0.32	0.044
神经侵犯	有	47	41(87.2)	0.111	1.06±0.34	0.284
神经侵犯	无	51	38(74.5)	0.111	1.10±0.35	0.284
淋巴结转移	有	54	50(92.6)	0.001	0.98±0.26	0.018
淋巴结转移	无	44	29(65.9)	0.001	1.12±0.39	0.018
HER-2	阳性	45	33(73.3)	0.093	0.99±0.22	0.074
HER-2	阴性	53	46(86.8)	0.093	1.06±0.25	0.074
Ki-67	高表达	38	28(73.7)	0.167	0.96±0.31	0.086
Ki-67	低表达	60	51(85.0)	0.167	1.05±0.32	0.086

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