Study on the expression of CRIP1 in gastric cancer and its relationship with clinicopathological index and prognosis

doi:10.3877/cma.j.issn.1674-3946.2024.02.015

Abstract

Abstract:

Objective

To investigate the expression and clinical significance of cysteine-rich intestinal protein-1 （CRIP1） in gastric cancer.

Methods

Sixty patients with gastric cancer admitted from January 2020 to December 2021 were selected as the study objects. Immunohistochemical method （IHC） and real-time quantitative fluorescent PCR （qRT-PCR） were used to detect the expression of CRIP1 protein and mRNA in gastric cancer tissue and corresponding adjacent normal tissue （>5 cm from the margin of cancer tissue）. SPSS 20.0 software was used to process the data， and the measurement data in clinicopathological characteristics were expressed as （

x ¯

±s）. Independent sample t test； The count data were represented by ［cases （%）］， in which Rank Sum test was used for rank count data and χ² test was used for other count data. Kaplan-Meier method was used to draw the survival curve， and Log-Rank was used to test the relationship between different expressions of CRIP1 and the prognosis of gastric cancer patients. ROC curve was drawn， AUC and Jorden index were calculated， and the optimal cut-off point was determined. P<0.05 was considered statistically significant.

Results

The positive expression rate of CRIP1 protein in gastric cancer tissues was significantly higher than that in adjacent normal tissues （83.3% vs.31.7%， P<0.05）， and the expression of CRIP1 mRNA in gastric cancer tissues was significantly up-regulated （P<0.05）. The high expression of CRIP1 was significantly correlated with invasion depth， pTMN stage and lymph node metastasis （P<0.05）. The cumulative overall survival rate and disease-free survival rate in the high expression group of CRIP1 were significantly lower than those in the low expression group （48.0% vs. 80.0%， 42.0% vs. 70.0%）， and the difference was statistically significant （Log-Rank χ²=4.008， 3.906， P=0.045， 0.048）. By drawing the ROC curve of CRIP1 mRNA relative expression level to predict the prognosis of gastric cancer， the AUC value was calculated as 0.865 （95%CI： 0.831-0.896），When the best cut-off value of CRIP1 mRNA expression level was 1.94， the sensitivity was 88.7%， the specificity was 76.5%， and the Yoden index was 0.652.

Conclusion

CRIP1 is highly expressed in gastric cancer tissues， and the high expression of CRIP1 is closely related to the depth of invasion， pTMN stage and lymph node metastasis of gastric cancer， which may be involved in the occurrence， development and metastasis of gastric cancer， and has certain predictive value for the prognosis of patients with gastric cancer.

Key words: Stomach Neoplasms, Cysteine-Rich Intestinal Protein1, Gene Expression, Prognosis

Lin Zhang, Ting Li. Study on the expression of CRIP1 in gastric cancer and its relationship with clinicopathological index and prognosis[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(02): 171-175.

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URL: https://zhpwkssxzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-3946.2024.02.015

https://zhpwkssxzz.cma-cmc.com.cn/EN/Y2024/V18/I02/171

Figures/Tables 6

临床资料	类别	例	临床资料	类别	例
性别	男	38(63.3)	年龄	<60	27(45.0)
性别	女	22(36.7)	年龄	≥60	33(55.0)
肿瘤位置	胃底	9(15.0)	浸润深度	T₁	7(11.7)
	胃体	20(33.3)		T₂	23(38.3)
	胃窦	31(51.7)		T₃	21(35.0)
肿瘤直径	<5 cm	39(65.0)		T₄	9(15.0)
肿瘤直径	≥5 cm	21(35.0)	pTMN分期	Ⅰ期	10(16.7)
分化程度	高分化	11(18.3)		Ⅱ期	16(26.7)
	中分化	19(31.7)		Ⅲ期	28(46.6)
	低分化	30(50.0)		Ⅳ期	6(10.0)
Borrmann分型	Ⅰ型	3(5.0)	淋巴结转移情况	有	38(63.3)
	Ⅱ型	10(16.7)	淋巴结转移情况	无	22(36.7)
	Ⅲ型	35(58.3)
	Ⅳ型	12(20.0)

组别	例数	CRIP1蛋白		CRIP1 mRNA表达( $x ¯$ ±s)
组别	例数	阳性[例(%)]	阴性[例(%)]	CRIP1 mRNA表达( $x ¯$ ±s)
胃癌组织	60	46(76.7)	14(23.3)	2.1±0.3
癌旁正常组织	60	19(31.7)	41(68.3)	0.8±0.1
χ²/t值		24.470	-	31.843
P值		0.000	-	0.000

组别	例数	CRIP1蛋白		CRIP1 mRNA表达( $x ¯$ ±s)
组别	例数	阳性[例(%)]	阴性[例(%)]	CRIP1 mRNA表达( $x ¯$ ±s)
胃癌组织	60	46(76.7)	14(23.3)	2.1±0.3
癌旁正常组织	60	19(31.7)	41(68.3)	0.8±0.1
χ²/t值		24.470	-	31.843
P值		0.000	-	0.000

临床病理特征	高表达组 (n=50)	低表达组(n=10)	χ²/Z值	P值
性别			0.014	0.905
男	32	6
女	18	4
年龄(岁)			0.485	0.486
<60	24	3
≥60	26	7
肿瘤直径(cm)			0.527	0.468
<5	34	5
≥5	16	5
肿瘤位置			0.401	0.818
胃底	8	1
胃体	17	3
胃窦	25	6
Borrmann分型			-0.491	0.623
Ⅰ型	2	1
Ⅱ型	8	2
Ⅲ型	30	5
Ⅳ型	10	2
浸润深度			-1.970	0.049
T₁	3	4
T₂	20	3
T₃	19	2
T₄	8	1
分化程度			0.705	0.703
高分化	10	1
中分化	16	3
低分化	24	6
pTMN分期			-3.098	0.002
Ⅰ期	10	0
Ⅱ期	15	1
Ⅲ期	23	5
Ⅳ期	2	4
淋巴结转移			7.593	0.006
有	36	2
无	14	8

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