Abstract:
Objective To explore the relationship between expression of TSHR, TSHR-mRNA and BRAFV600E gene mutation in papillary thyroid cancer.
Method A total of 122 patients with papillary thyroid cancer were enrolled in this study. PCR was applied to detect gene mutation of BRAFV600E in papillary thyroid cancers. All the patients were divided into BRAFV600E gene mutation group and BRAFV600E gene wild-type group. Immunohistochemical assay were used to detect expression of TSHR in papillary thyroid cancers. Western blotting and Real-time quantitative PCR (RT-PCR) were used to verify the results. Statistical analysis was performed by using SPSS17.0 software. Chi-squared test and Independent sample t test were applied to analyze the relationship between the expressions of TSHR, TSHR-mRNA and BRAFV600E gene mutation. A P value <0.05 was considered as statistical significance.
Result The expression levels of TSHR and TSHR-mRNA in papillary thyroid cancers was significant decreased (P<0.05), and the expression of TSHR and TSHR-mRNA in papillary thyroid cancers were decreased more obviously in BRAFV600E gene mutation group than those in BRAFV600E gene wild-type group.
Conclusion BRAFV600E gene mutation is associated with the lower expression of TSHR in papillary thyroid cancers.
Key words:
Thyroid Neoplasms,
Carcinoma, Papillary,
Receptors, Thyrotropin
Shaolong Hao, Jihong Ma, Xincheng Liu, Huanjie Chen, Haitao Zheng, Xicheng Song. Clinical investigation of the expression of TSHR and BRAFV600E gene mutation in papillary thyroid carcinoma[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2018, 12(06): 468-471.