Abstract:

Objective

The mouse model of postoperative anastomotic leakage of digestive tract was established.

Methods

From June 2023 to December 2023, 30 C57BL/6 mice were randomly divided into two groups: 6 mice in the control group were performed laparotomy only; In the experimental group of 24, the intestinal tube was cut from the vascularized area of the colon and the blood vessels supplying this section were ligation, then anastomosis was performed, and the operation time was recorded.Two days after operation,a laparotomy was performed to investigate the morphological changes in the abdominal cavity of the mice, and the tissues around the anastomosis were collected to further observe the histological changes of the anastomotic leakage.Image J and Graph Pad Prism 9.5 software were used for statistical analysis.Independent sample t test was used for comparison between the two groups, and P＜0.05 was considered statistically significant.

Results

The average construction time of the model was 25min, and the survival rate of the mice was 75%.Methylene blue perfusion test showed leakage of intestinal tube.Laparotomy exploration showed that there was slight adhesion in the anastomosis, the adhesion part could be separated, and there was leakage in the intestinal tube.Compared with the control group, hematoxylin-eosin staining showed muscle discontinuity at the anastomosis in the experimental group.Western Blot showed that the expression of inflammatory factors in the same part of the tissue was significantly different at the protein level.F4/80 immunohistochemistry showed a large number of macrophage infiltrates at the site of muscle break.

Conclusion

Through this method, a mouse model of postoperative anastomotic leakage of digestive tract can be successfully constructed.

Key words: Surgical Procedures, Operative, Postoperative Complications, Anastomotic Leak, Models, Animals