Relationship between DNMT3B and prognosis of breast cancer and its biological mechanism

doi:10.3877/cma.j.issn.1674-3946.2024.06.010

Abstract

Abstract:

Objective

To analyze the expression of DNA methyltransferase 3B (DNMT3B) in breast cancer and its influence on prognosis, and explore its role in the occurrence and development of breast cancer and potential targets.

Methods

Analyzing TCGA breast cancer database through R language program, Online databases such as UALCAN, Starbase database, cBioPortal, GeneMANIA and STRING were used to analyze DNMT3B expression, diagnostic and prognostic value, tumor immune microenvironment, GO function, KEGG signaling pathway and protein interaction in breast cancer. The expression difference of DNMT3B between common breast cancer cell lines and normal breast epithelial cells was detected by QRT-PCR.

Results

Online database analysis and validation experiments suggest that DNMT3B is highly expressed in breast cancer, and its high expression is not conducive to the survival of breast cancer patients, suggesting that DNMT3B may have diagnostic value. Through GO function, KEGG signaling pathway and protein interaction, and tumor immune microenvironment analysis, DNMT3B is involved in multiple mechanisms of breast cancer occurrence and development, and may become a new therapeutic target.

Conclusion

DNMT3B can promote the occurrence of breast cancer and affect the progression of the disease, and may be a potential tumor marker and therapeutic target for breast cancer.

Key words: Breast Neoplasms, DNA methyltransferase B, Tumor Immune Microenvironment, Prognosis

Zhizhao Zhang, Rui Wang, Pingping Gao, Chengfang Wang, Cheng Wang, Xiaowei Qi. Relationship between DNMT3B and prognosis of breast cancer and its biological mechanism[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(06): 624-629.

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Figures/Tables 8

References 21

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MiRNAname	geneName	R-value	p-value
hsa-miR-29c-3p	DNMT3B	-0.34	9.36E-31
hsa-miR-30a-5p	DNMT3B	-0.328	1.33E-28
hsa-let-7b-5p	DNMT3B	-0.314	2.73E-26
hsa-let-7a-5p	DNMT3B	-0.258	5.71E-18
hsa-miR-375	DNMT3B	-0.184	1.04E-09
hsa-miR-1-3p	DNMT3B	-0.138	5.40E-06
hsa-miR-135a-5p	DNMT3B	-0.135	8.25E-06
hsa-miR-195-5p	DNMT3B	-0.119	9.02E-05
hsa-miR-29a-3p	DNMT3B	-0.112	2.26E-04
hsa-miR-29b-3p	DNMT3B	-0.106	4.51E-04
hsa-miR-125a-5p	DNMT3B	-0.097	1.43E-03
hsa-miR-26a-5p	DNMT3B	-0.093	2.16E-03
hsa-miR-26b-5p	DNMT3B	-0.062	4.15E-02
hsa-miR-628-5p	DNMT3B	0.052	8.52E-02
hsa-miR-361-3p	DNMT3B	0.058	5.67E-02
hsa-miR-95-3p	DNMT3B	0.07	2.04E-02
hsa-miR-30d-5p	DNMT3B	0.104	5.77E-04
hsa-miR-148a-3p	DNMT3B	0.115	1.41E-04
hsa-miR-21-5p	DNMT3B	0.117	1.10E-04
hsa-miR-885-5p	DNMT3B	0.118	9.63E-05
hsa-miR-328-3p	DNMT3B	0.139	4.55E-06
hsa-miR-140-5p	DNMT3B	0.145	1.66E-06
hsa-miR-222-3p	DNMT3B	0.152	4.95E-07
hsa-let-7g-5p	DNMT3B	0.156	2.51E-07
hsa-miR-30e-5p	DNMT3B	0.199	3.75E-11

MiRNAname	geneName	R-value	p-value
hsa-miR-29c-3p	DNMT3B	-0.34	9.36E-31
hsa-miR-30a-5p	DNMT3B	-0.328	1.33E-28
hsa-let-7b-5p	DNMT3B	-0.314	2.73E-26
hsa-let-7a-5p	DNMT3B	-0.258	5.71E-18
hsa-miR-375	DNMT3B	-0.184	1.04E-09
hsa-miR-1-3p	DNMT3B	-0.138	5.40E-06
hsa-miR-135a-5p	DNMT3B	-0.135	8.25E-06
hsa-miR-195-5p	DNMT3B	-0.119	9.02E-05
hsa-miR-29a-3p	DNMT3B	-0.112	2.26E-04
hsa-miR-29b-3p	DNMT3B	-0.106	4.51E-04
hsa-miR-125a-5p	DNMT3B	-0.097	1.43E-03
hsa-miR-26a-5p	DNMT3B	-0.093	2.16E-03
hsa-miR-26b-5p	DNMT3B	-0.062	4.15E-02
hsa-miR-628-5p	DNMT3B	0.052	8.52E-02
hsa-miR-361-3p	DNMT3B	0.058	5.67E-02
hsa-miR-95-3p	DNMT3B	0.07	2.04E-02
hsa-miR-30d-5p	DNMT3B	0.104	5.77E-04
hsa-miR-148a-3p	DNMT3B	0.115	1.41E-04
hsa-miR-21-5p	DNMT3B	0.117	1.10E-04
hsa-miR-885-5p	DNMT3B	0.118	9.63E-05
hsa-miR-328-3p	DNMT3B	0.139	4.55E-06
hsa-miR-140-5p	DNMT3B	0.145	1.66E-06
hsa-miR-222-3p	DNMT3B	0.152	4.95E-07
hsa-let-7g-5p	DNMT3B	0.156	2.51E-07
hsa-miR-30e-5p	DNMT3B	0.199	3.75E-11

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