BANCR regulates HIF-1α to promote microlymphangiogenesis in pancreatic cancer under hypoxic microenvironment

doi:10.3877/cma.j.issn.1674-3946.2021.03.009

Abstract

Abstract:

Objective

To study the regulation of BANCR on pancreatic cancer HIF-1α and its effect on microlymphangiogenesis in pancreatic cancer cell-lines under hypoxic conditions.

Methods

qRT-PCR was used to detect the expression of BANCR in pancreatic cancer cell-lines (SW1990, PANC-1) under normoxia and hypoxia respectively. The expression of BANCR of pancreatic cancer cell-lineswere interfered by siRNA, and were 3D co-cultured with human lymphatic endothelial cells (HDLEC) under normoxia and hypoxia conditions, and the microlymphangiogenesis density (MLVD) were counted. qRT-PCR was used to detect the relative expression of HIF-1α protein and its mRNA in pancreatic cancer cell-lines of si-BANCR group and si-NC group.

Results

Compared with the normoxia group, significantly higher level of BANCR expression in pancreatic cancer cell-lines were observed in the hypoxia group (P<0.01). Once BANCR expression were interfered, compared with the normoxia group, the level of MLVD were significantly increased in the NC group and si-BANCR group under hypoxic conditions (P<0.001). Silencing the expression of BANCR could significantly reduce the level of MLVD in pancreatic cancer cell-lines (P<0.05); Compared with the si-BANCR group, both the mRNA and protein expression levels of HIF-1α were significantly increased in the si-NC group under hypoxic conditions.

Conclusion

The expression of BANCR in pancreatic cancer cell-lines (SW1990, PANC-1) increased under hypoxic conditions, which could up-regulate the expression levels of HIF-1α at the transcription and translation, which might play an important role in pancreatic cancer microlymphangiogenesis and lymph node metastasis.

Key words: Pancreatic neoplasms, Cell hypoxia, Long non-coding RNA

Shaolong Hao, Yu Ji, Hao Sun, Haowei Shi, Jihong Ma, wei Han. BANCR regulates HIF-1α to promote microlymphangiogenesis in pancreatic cancer under hypoxic microenvironment[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2021, 15(03): 262-266.

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名称	上游引物
GAPDH	5’-TCAAGAAGGTGGTGAAGCAGG-3’
BANCR	5’-GAGCCTTGCCAGTTCCATTT-3’
HIF-1α	5’-ACTTCTGGATGCTGGTGATTTG-3’
Si-BANCR	5’-GGUGTGGCGUCTUTUGCUUTT-3’
名称	下游引物
GAPDH	5’-TCAAAGGTGGAGGAGTGGGT-3’
BANCR	5’-TGCAGAGGTGAGATTCAGGT-3’
HIF-1α	5’-GCTTCGCTGTGTGTTTTGTTCT-3’
Si-BANCR	5’-AAGCAAAAGACGCCACACCTT-3’

名称	上游引物
GAPDH	5’-TCAAGAAGGTGGTGAAGCAGG-3’
BANCR	5’-GAGCCTTGCCAGTTCCATTT-3’
HIF-1α	5’-ACTTCTGGATGCTGGTGATTTG-3’
Si-BANCR	5’-GGUGTGGCGUCTUTUGCUUTT-3’
名称	下游引物
GAPDH	5’-TCAAAGGTGGAGGAGTGGGT-3’
BANCR	5’-TGCAGAGGTGAGATTCAGGT-3’
HIF-1α	5’-GCTTCGCTGTGTGTTTTGTTCT-3’
Si-BANCR	5’-AAGCAAAAGACGCCACACCTT-3’

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