目的

研究电子转移黄素蛋白A（ETFA）和CD34在乳腺癌中的表达及与临床病理参数和预后的关系。

方法

回顾性收集2020年12月至2024年2月行手术治疗的女性乳腺癌患者115例为研究对象，术后均随访36个月。采用统计学软件SPSS 22.0分析数据。符合正态分布的计量资料用（x ± s）表示，行独立样本t检验；计数资料用［例（%）］表示采用χ²检验；Spearson分析乳腺癌组织中ETFA和CD34阳性表达与不同病理参数的相关性；采用Kaplan-Meier生存曲线分析乳腺癌患者3年生存率。P＜0.05为差异有统计学意义。

结果

ETFAmRNA和CD34mRNA在乳腺癌组织和癌旁正常组织表达有显著差异（P＜0.05）；乳腺癌组织中ETFA和CD34阳性表达率分别为73.9%和77.4%，癌旁正常组织中分别为25.2%和9.6%，差异均有统计学意义（P＜0.05）；乳腺癌组织中ETFA和CD34阳性表达率在肿瘤分期、分化程度、淋巴结转移中差异有统计学意义（P＜0.05）；Spearson相关性分析乳腺癌组织中ETFA和CD34阳性表达与肿瘤分期、淋巴结转移呈正相关，与肿瘤分化程度呈负相关。ETFA阳性乳腺癌患者3年生存率为55.3%，低于ETFA阴性患者的59.0%（P=0.021）；CD34阳性乳腺癌患者的3年生存率为51.6%，低于CD34阴性患者的62.7%（P=0.000）。

结论

乳腺癌组织中ETFA mRNA和CD34mRNA呈高表达，与肿瘤恶化发展和患者不良预后密切相关，以期成为评估乳腺癌患者病情严重程度的标志物。

Objective

To investigate the relationship between the expression of electron transfer flavin protein A （ETFA） and Hematopoietic progenitor cell antigen （CD34） and clinicopathological parameters and prognosis in breast cancer.

Methods

A total of 115 female breast cancer patients who underwent surgical treatment from December 2020 to February 2024 were retrospectively collected and followed up for 36 months.Statistical software SPSS 22.0 was used to analyze the data.The measurement data conforming to the normal distribution were represented by （x ± s）， and the independent sample t was compared.The counting data were represented by［ cases （%）］ and compared by χ² test.The positive expression of ETFA and CD34 in breast cancer tissues was correlated with different pathological parameters by Spearson analysis.Kaplan-Meier survival curve was used to analyze the 3-year survival rate of breast cancer patients.P＜0.05 indicated that the difference was statistically significant.

Result

There were significant differences in the expression of ETFA and CD34mRNA in adenocarcinoma tissues and adjacent tissues （P＜0.05）.The positive expression rates of ETFA and CD34 in breast cancer tissues were 73.9% and 77.4%， respectively， and the expression rates in adjacent tissues were 25.2% and 9.6%， respectively， with statistical significance （P＜0.05）.The positive expression rates of ETFA and CD34 in breast cancer tissues were significantly different in tumor stage， differentiation degree and lymph node metastasis （P＜0.05）.Spearson correlation analysis showed that the positive expression of ETFA and CD34 in breast cancer tissues was positively correlated with tumor stage and lymph node metastasis， and negatively correlated with tumor differentiation.The 3-year survival rate of ETFA-positive breast cancer patients was 55.3%， lower than that of ETFA-negative breast cancer patients （59.0%， P=0.021）.The 3-year survival rate for patients with CD34-positive breast cancer was 51.6%， compared with 62.7% for patients with CD34-negative breast cancer （P=0.000）.

Conclusion

The high expression of ETFA mRNA and CD34mRNA in breast cancer tissues is closely related to the development of tumor deterioration and poor prognosis of patients，with a view to becoming a marker to evaluate the severity of breast cancer patients.