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中华普外科手术学杂志(电子版)

中华普外科手术学杂志(电子版) ›› 2020, Vol. 14 ›› Issue (06) : 600 -603. doi: 10.3877/cma.j.issn.1674-3946.2020.06.019

所属专题： 文献；

论著

长链非编码RNA HIF1A-AS2在结直肠癌组织中的表达及临床意义
高永涛1, 王领2,†(), 刘涛3, 袁江涛3, 白浪3, 李娟3, 强跳4   
  1. 1. 716000　陕西延安，延安大学附属医院普通外科
    2. 712000　西安，陕西省核工业215医院
    3. 716000　陕西延安，延安大学附属医院手术室
    4. 716000　陕西延安，延安大学附属医院检验科
  • 收稿日期:2020-03-04 出版日期:2020-12-26
  • 通信作者: 王领

Expression and clinical significance of Lnc RNA HIF1A-AS2 in colorectal cancer tissues

Yongtao Gao1, Ling Wang2,†(), Tao Liu3, Jiangtao Yuan3, Lang Bai3, Juan Li3, Tiao Qiang4   

  1. 1. Department of General Surgery, the Affiliated Hospital of Yan'an University, Shaanxi 716000, China
    2. The 215 Hospital, Nuclear Industry of Shaanxi province, Shaanxi 712000, China
    3. Operating Room, the Affiliated Hospital of Yan'an University, Shaanxi 716000, China
    4. Department of laboratory, the Affiliated Hospital of Yan'an University, Shaanxi 716000, China
  • Received:2020-03-04 Published:2020-12-26
  • Corresponding author: Ling Wang
  • About author:
    Corresponding author: Wang Ling, Email:
  • Supported by:
    Shaanxi Provincial Natural Science Fundamental Research Program(2015JM7062); Project of Shaanxi Province Health and Family Planning Commission Foundation(2015KW-02); Shaanxi Provincial Social development science and technology program(2020SF091)
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高永涛, 王领, 刘涛, 袁江涛, 白浪, 李娟, 强跳. 长链非编码RNA HIF1A-AS2在结直肠癌组织中的表达及临床意义[J/OL]. 中华普外科手术学杂志(电子版), 2020, 14(06): 600-603.

Yongtao Gao, Ling Wang, Tao Liu, Jiangtao Yuan, Lang Bai, Juan Li, Tiao Qiang. Expression and clinical significance of Lnc RNA HIF1A-AS2 in colorectal cancer tissues[J/OL]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2020, 14(06): 600-603.

目的

检测LncRNA HIF1A-AS2在结直肠癌组织中的表达，并探讨其临床意义。

方法

选取2012年12月至2015年12月行结直肠癌患者根治术的114例结直肠癌患者临床病理资料；收集其癌组织及相对应的癌旁组织(距病灶2 cm)和正常组织(距癌灶边缘>5 cm)标本。通过qRT-PCR检测结直肠癌组织、癌旁组织及癌旁正常组织中HIF1A-AS2相对表达水平。取114例入组患者HIF1A-AS2表达水平的平均数，将HIF1A-AS2表达水平大于等于平均数的89例纳入高表达组，其余纳入低表达组；分析HIF1A-AS2表达与临床病理特征之间的关系；Kaplan-Meier生存曲线分析两组患者术后5年生存情况，Log-rank检验比较生存差异；COX比例风险回归模型分析预后不良的独立危险因素；P<0.05为差异有统计学意义。

结果

结直肠癌组织中HIF1A-AS2相对表达量高于癌旁组织及正常组织(P<0.001)。HIF1A-AS2表达与临床分期及组织分化程度显著相关(P<0.05)。HIF1A-AS2高表达组患者术后5年总生存率低于HIF1A-AS2低表达组(Log-rank χ2＝5.037, P＝0.025)。COX多因素分析显示淋巴结转移、临床分期Ⅳ期、中-低分化、有远处转移及HIF1A-AS2显著高表达是影响结直肠癌患者预后不良的独立危险因素(P<0.05)。

结论

结直肠癌组织中HIF1A-AS2显著高表达，与临床病理及预后密切相关，是影响结直肠癌患者预后不良的危险因素之一。

关键词: 结直肠肿瘤, LncRNA HIF1A-AS2, 基因表达, 预后
Objective

To investigate the expression of LncRNA HIF1A-AS2 in colorectal cancer tissues and to explore its clinical significance.

Methods

Clinicopathological data of 114 patients, who were with colorectal cancer from December 2012 to December 2015 and underwent radical resection, were collected, including samples of the cancer tissue and corresponding adjacent tissues (2 cm away from the lesion) and adjacent normal tissues (5 cm away from the edge of the cancer lesion). The relative expression levels of HIF1A-AS2 in colorectal cancer tissues, adjacent tissues and adjacent normal tissues were detected by qRT-PCR. The average expression level of HIF1A-AS2 in 114 enrolled patients was evaluated, and 89 patients with HIF1A-AS2 expression level greater than or equal to the average were divided into the high-expression group, while the rest were divided into the low-expression group. The relationship between HIF1A-AS2 expression and clinicopathological features was analyzed. Kaplan-meier survival curve was usedto analyze the 5-years survival of patients in two groups by using log-rank test. COX proportional risk regression model were used to analyze the independent risk factors for prognosis. A P value of < 0.05 was considered as statistically significant difference.

Results

The relative expression of HIF1A-AS2 in colorectal cancer tissues was higher than that in adjacent tissues and normal tissues (P<0.001). HIF1A-AS2 expression was significantly correlated with clinical stage and degree of differentiation in cancer tissue (P<0.05). The 5-year overall survival rate of the HIF1A-AS2 high-expression group was lower than that of the HIF1A-AS2 low-expression group (log-rank χ2=5.037, P=0.025). COX multi-factor analysis showed that lymph node metastasis, clinical stage Ⅳ, in poorly differentiated, with distant metastasis and HIF1A AS2 significantly high expression were the independent risk factors influencing the prognosis of patients with colorectal cancer (P<0.05).

Conclusion

HIF1A-AS2 is highly expressed in colorectal cancer tissues, which is closely related to clinicopathology and prognosis, and is one of the risk factors for poor prognosis of patients with colorectal cancer.

Key words: Colorectal neoplasms, LncRNA HIF1A-AS2, Gene expression, Prognosis
表1 HIF1A-AS2的表达水平与114例结直肠癌患者临床病理参数的关系[(±s)，例]
临床病理特征 例数 HIF1A-AS2表达 χ 2/t P
高(n＝69) 低(n＝45)
年龄(岁) - 59.1±7.3 61.4±10.4 1.360 0.088
? ≥60 65 37 28 ? ?
? <60 49 32 17 0.822 0.365
性别 ? ? ? ? ?
? 男 62 38 24 ? ?
? 女 52 31 21 0.033 0.855
淋巴转移 ? ? ? ? ?
? 是 51 34 17 ? ?
? 否 63 35 28 1.456 0.228
分化程度 ? ? ? ? ?
? 高分化 74 38 36 ? ?
? 中-低分化 40 31 9 7.431 0.006
TNM分期 ? ? ? ? ?
? Ⅰ期 36 15 21 ? ?
? Ⅱ期 37 22 15 ? ?
? Ⅲ期 21 15 6 ? ?
? Ⅳ期 20 16 4 9.489 0.023
肿瘤最大径(cm) - 4.2±2.8 4.8±1.8 1.276 0.102
? ≥5 40 24 16 ? ?
? <5 74 45 29 0.007 0.933
病变部位 ? ? ? ? ?
? 结肠 44 30 14 ? ?
? 直肠 70 39 31 1.758 0.185
远处转移 ? ? ? ? ?
? M0 84 46 38 ? ?
? M1 30 17 13 0.032 0.857
图1 qRT-PCR检测114例结直肠癌组织中HIF1A-AS2表达图注：按照"-ΔΔCT"大小依次排列，黑色低于正常组织，红色高于正常组织。
图2 结直肠癌组织、癌旁组织及正常组织中HIF1A-AS2表达[注：较正常组织比，**P<0.001]
图3 114例结直肠癌患者HIF1A-AS2高、低表达组5年总生存率分析
表2 影响结直肠癌患者预后的生存分析
临床病理特征 单因素分析
HR (95% CI) P
年龄(≥60岁) 0.156(0.743～1.799) 0.521
性别(男) 1.348(0.824～2.203) 0.234
淋巴转移(是) 2.865(1.801～6.854) 0.033
TNM (Ⅳ期) 1.912(2.627～5.780) 0.002
肿瘤最大径(≥5 cm) 1.943(0.982～3.831) 0.354
分化程度(中-低分化) 1.829(1.149～2.911) 0.014
HIF1A-AS2高表达 1.629(1.371～8.159) 0.021
病变部位(结肠) 2.103(0.716～6.205) 0.201
远处转移(M1) 1.348(1.824～2.230) 0.003
临床病理特征 多因素分析
HR (95% CI) P
年龄(≥60岁) - -
性别(男) - -
淋巴转移(是) 2.184(1.473～4.012) 0.018
TNM (Ⅳ期) 1.654(1.124～2.435) 0.011
肿瘤最大径(≥5 cm) - -
分化程度(中-低分化) 1.952(2.568～8.700) 0.017
HIF1A-AS2高表达 1.721(1.116～2.656) 0.014
病变部位(结肠) - -
远处转移(M1) 1.537(1.007～2.345) 0.046
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