Mechanism investigation of magnolol on proliferation and apoptosisof gastric cancercells

doi:10.3877/cma.j.issn.1674-3946.2019.03.015

Abstract

Abstract:

Objective

To investigatethemechanismofmagnolol on proliferation and apoptosis of gastric cancer cells.

Methods

SGC-7901 cells werecultured with 0 (control), 20, 40, 80 μmol/Lmagnolol for 48 hrespectively. Cell viability was measured by using MTT assay. Cell apoptosis and Cell cycle was measured by usingflow cytometry. The expression of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), cysteinyl aspartate specific proteinase 3 (cleaved caspase 3 ), CyclinD1, CyclinE and cyclin dependent kinase 2 (CDK2) protein were measured by western blotting. All the data were analyzed by using SPSS 17.0 software. Measurement data, including cell proliferation inhibition rate, cell apoptosis, cell cycle and protein expression, were expressed as (±s), and were examinedby using t test. A P value<0.05 was considered as statistically significant difference.

Results

Compared with control group, SGC-7901 cells with 20, 40, 80 μmol/L magnolol, exhibited decreased cell viability, increased apoptotic rate, arrested in the G1 phage, with down-regulated expression of Bcl-2, CyclinD1, CyclinE and CDK2, and up-regulated expression of Bax, cleaved caspase 3 (P<0.01).

Conclusion

Magnolol could inhibit SGC-7901 cells proliferation and induce cell apoptosis via regulation the expression of cell cycle protein and cell apoptotic related protein.

Key words: Stomach neoplasms, Cell proliferation, Apoptosis, Magnolol

Siman Lou, Gang Du, Langhamu Ze, Zhi Ding. Mechanism investigation of magnolol on proliferation and apoptosisof gastric cancercells[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2019, 13(03): 259-261.

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Figures/Tables 4

References 15

[1]	邹文斌，吴浩，蔡全才，等．胃癌危险因素研究进展[J]．中国实用内科杂志，2014，34(4)：415-420.
[2]	李雪平，惠起源．胃癌发生部位变化的流行病学研究进展[J]．中国医药导报，2014，11(17)：160-162.
[3]	廖毅，邓媛，傅建伟．胃癌分子机制的研究进展[J]．中国肿瘤，2014，23(1)：58-62.
[4]	赵益，王高玉，戈淑超，等．中医药对胃癌预防的研究进展[J]．中华中医药杂志，2017，32(3)：1162-1164.
[5]	冯颖，吴成亚，李杰．中医药治疗胃癌的优势及可能机制研究进展[J]. 辽宁中医杂志，2017，44(1)：200-203.
[6]	马梦雨，韩克起．中医药治疗胃癌研究进展[J]. 中医学报，2015，30(6)：777-779.
[7]	张淑洁，钟凌云. 厚朴化学成分及其现代药理研究进展[J]. 中药材，2013，36(5)：838-843.
[8]	杨丽华，马春，李淑玲．厚朴治疗血管性痴呆的研究进展[J]. 中国老年学杂志，2016，36(9)：2290-2291.
[9]	黄杰，李莎，伍春莲．厚朴酚抗肿瘤机制的研究进展[J]. 天然产物研究与开发，2016，28(4)：637-641.
[10]	Xu HL, Tang W, Du GH, et al. Targeting apoptosis pathways in cancer with magnolol and honokiol, bioactive constituents of the bark of Magnolia officinalis[J]. Drug Discov Ther, 2011, 5(5): 202-210.
[11]	Shen J, Ma H, Zhang T, et al. Magnolol Inhibits the Growth of Non－Small Cell Lung Cancer via Inhibiting Microtubule Polymerization[J]. Cell Physiol Biochem, 2017, 42(5): 1789-1801.
[12]	Mei C, He SS, Yin P, et al. Magnolol pretreatment attenuates heat stress－induced IEC－6 cell injury[J]. J Zhejiang Univ Sci B, 2016, 17(6): 413-424.
[13]	Bayraktar S, Rocha LC. Emerging cell－cycle inhibitors for pancreatic cancer therapy[J]. Expert Opin Emerg Drugs, 2012, 17(4): 571-582.
[14]	刘雷，陈国昌，宋振云，等. Runx3、Smad4、Cdk2、p21在胃癌中的表达及其临床意义[J]. 胃肠病学，2015，20(10)：597-601.
[15]	Han P, Wang QL, Zhang X. Expression of TRAP1 in gastric cancer tissue and its correlation with malignant biology[J]. Asian Pac J Trop Med, 2016, 9(1): 67-71.

组别(μmol/L)	24 h	48 h	72 h
对照组	0	0	0
20	5.3±0.5^a	8.4±0.8^a	18.4±1.8^a
40	12.1±1.2^a	20.9±2.4^a	38.4±3.7^a
80	19.0±1.9^a	38.5±3.5^a	59.4±4.9^a

组别(μmol/L)	24 h	48 h	72 h
对照组	0	0	0
20	5.3±0.5^a	8.4±0.8^a	18.4±1.8^a
40	12.1±1.2^a	20.9±2.4^a	38.4±3.7^a
80	19.0±1.9^a	38.5±3.5^a	59.4±4.9^a

组别(μmol/L)	细胞凋亡率(%)		细胞周期(%)
组别(μmol/L)	早期凋亡	晚期凋亡	G1	S	G2
对照组	2.3±0.2	3.3±0.3	45.2±4.5	32.3±3.1	22.5±2.3
20	8.3±0.8^a	6.7±0.7^a	50.7±5.1^a	28.2±2.8^a	21.1±2.1^a
40	14.9±1.8^a	12.3±1.2^a	56.5±5.6^a	24.4±2.44^a	19.1±1.9^a
80	22.4±2.6^a	17.3±1.8^a	63.4±6.3^a	20.6±2.1^a	16.0±1.6^a

组别(μmol/L)	细胞凋亡率(%)		细胞周期(%)
组别(μmol/L)	早期凋亡	晚期凋亡	G1	S	G2
对照组	2.3±0.2	3.3±0.3	45.2±4.5	32.3±3.1	22.5±2.3
20	8.3±0.8^a	6.7±0.7^a	50.7±5.1^a	28.2±2.8^a	21.1±2.1^a
40	14.9±1.8^a	12.3±1.2^a	56.5±5.6^a	24.4±2.44^a	19.1±1.9^a
80	22.4±2.6^a	17.3±1.8^a	63.4±6.3^a	20.6±2.1^a	16.0±1.6^a

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