The relationship between the expression of ELK3 and clinicopathologic features and prognosis in gastric and paracancer tissues

doi:10.3877/cma.j.issn.1674-3946.2024.02.011

Abstract

Abstract:

Objective

To analyze the relationship between the expression of transcription factor ELK3 (ELK3) and the clinicopathologic features and prognosis in gastric and paracancer tissues.

Methods

Samples of cancer tissues and corresponding paracancer tissues (3~5 cm away from tumor margin) from 60 patients with gastric cancer who underwent surgical treatment from January 2019 to June 2021 were selected for study. The expression of ELK3 in gastric cancer tissues and adjacent tissues was detected by immunohistochemical staining. SPSS 21.0 software was used to analyze the data, and the count data were presented as examples. χ² test was used for comparison between groups. Kaplan-Meier survival function was used to analyze the relationship between ELK3 and prognosis. Cox regression model was used to analyze the risk factors affecting prognosis and survival of patients with gastric cancer. P<0.05 meant the difference was statistically significant.

Results

The positive expression rate of ELK3 in gastric cancer tissues was significantly higher than that in paracancer tissues (χ²=11.422, P=0.001). The positive expression rate of ELK3 in gastric cancer tissues was significantly correlated with lymph node metastasis, tumor stage, degree of differentiation and pathological classification (P<0.05). All 60 patients with gastric cancer were followed up for a median of 25 months.The cumulative overall survival rate of ELK3-positive group was significantly lower than that of ELK3-negative group (Log-Rank χ²=4.192, P=0.041). Lymph node metastasis, tumor stage, degree of differentiation, nerve invasion, postoperative chemoradiotherapy and positive expression of ELK3 were independent risk factors affecting the prognosis of gastric cancer patients (P<0.05).

Conclusion

The significant positive expression of ELK3 in gastric cancer tissues is closely related to pathological characteristics such as lymph node metastasis, tumor stage, differentiation degree, pathological type and patient prognosis. It is an independent risk factor affecting the prognosis of cancer patients, and may become a potential biomarker for the prognosis evaluation of gastric cancer.

Key words: Neoplasm of Stomach, Gastrectomy, Laparoscopic, The Transcription Factor ELK3, Immunohistochemical Staining, Clinicopathology, Prognosis

Zhenghong Liu, Fengli Wang, Yajun Ji, Jia Gao. The relationship between the expression of ELK3 and clinicopathologic features and prognosis in gastric and paracancer tissues[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(02): 155-159.

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Figures/Tables 4

References 19

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病理特征		例数	阳性（n=46）	χ²值	P值
性别	男	36	27（58.7）	0.140	0.709
性别	女	24	19（41.3）	0.140	0.709
年龄（岁）	<60	23	17（37.0）	0.158	0.691
年龄（岁）	≥60	37	29（63.0）	0.158	0.691
肿瘤大小（cm）	<4	41	31（67.4）	0.081	0.776
肿瘤大小（cm）	≥4	19	15（32.6）	0.081	0.776
浸润深度	T₁	6	4（8.7）	1.124	0.771
	T₂	25	19（41.3）
	T₃	20	15（32.6）
	T₄	9	8（17.4）
淋巴结转移	有	28	17（37.0）	7.468	0.006
淋巴结转移	无	32	29（63.0）	7.468	0.006
肿瘤分期	Ⅰ-Ⅱ期	24	15（32.6）	4.488	0.034
肿瘤分期	Ⅲ-Ⅳ期	36	31（67.4）	4.488	0.034
分化程度	高分化	14	8（17.4）	6.591	0.037
	中分化	21	15（32.6）
	低分化	25	23（50.0）
病理分型	管状腺癌	17	9（19.6）	7.944	0.047
	黏液腺癌	4	4（8.7）
	乳头状腺癌	26	22（47.8）
	印戒细胞癌	13	11（23.9）
神经侵犯	是	22	18（31.9）	0.515	0.473
神经侵犯	否	38	28（60.9）	0.515	0.473
脉管侵犯	是	42	33（28.3）	0.284	0.594
脉管侵犯	否	18	13（71.7）	0.284	0.594
术后放化疗	是	33	27（58.7）	1.088	0.297
术后放化疗	否	27	19（41.3）	1.088	0.297

病理特征		例数	阳性（n=46）	χ²值	P值
性别	男	36	27（58.7）	0.140	0.709
性别	女	24	19（41.3）	0.140	0.709
年龄（岁）	<60	23	17（37.0）	0.158	0.691
年龄（岁）	≥60	37	29（63.0）	0.158	0.691
肿瘤大小（cm）	<4	41	31（67.4）	0.081	0.776
肿瘤大小（cm）	≥4	19	15（32.6）	0.081	0.776
浸润深度	T₁	6	4（8.7）	1.124	0.771
	T₂	25	19（41.3）
	T₃	20	15（32.6）
	T₄	9	8（17.4）
淋巴结转移	有	28	17（37.0）	7.468	0.006
淋巴结转移	无	32	29（63.0）	7.468	0.006
肿瘤分期	Ⅰ-Ⅱ期	24	15（32.6）	4.488	0.034
肿瘤分期	Ⅲ-Ⅳ期	36	31（67.4）	4.488	0.034
分化程度	高分化	14	8（17.4）	6.591	0.037
	中分化	21	15（32.6）
	低分化	25	23（50.0）
病理分型	管状腺癌	17	9（19.6）	7.944	0.047
	黏液腺癌	4	4（8.7）
	乳头状腺癌	26	22（47.8）
	印戒细胞癌	13	11（23.9）
神经侵犯	是	22	18（31.9）	0.515	0.473
神经侵犯	否	38	28（60.9）	0.515	0.473
脉管侵犯	是	42	33（28.3）	0.284	0.594
脉管侵犯	否	18	13（71.7）	0.284	0.594
术后放化疗	是	33	27（58.7）	1.088	0.297
术后放化疗	否	27	19（41.3）	1.088	0.297

影响因素	单因素		多因素
影响因素	HR（95%CI）值	P值	HR（95%CI）值	P值
性别（男）	0.739（0.523~1.051）	0.682	-	-
年龄（≥60岁）	0.284（0.061~1.206）	0.349	-	-
肿瘤大小（≥4 cm）	1.979（1.621~2.417）	0.008	1.133（0.433~2.948）	0.732
浸润深度（T3-T4）	1.852（1.004~3.414）	0.047	0.706（0.345~1.443）	0.340
淋巴结转移（有）	1.633（1.112~2.401）	0.000	2.219（1.223~4.026）	0.000
肿瘤分期（Ⅲ-Ⅳ期）	1.258（1.057~3.035）	0.034	1.067（1.021~3.143）	0.039
分化程度（低分化）	2.301（1.331~3.979）	0.003	1.466（1.074~2.201）	0.028
病理分型	0.898（0.735~1.104）	0.133	-	-
神经侵犯（是）	2.271（1.276~4.338）	0.007	1.273（1.143~3.086）	0.031
脉管侵犯（是）	1.413（0.823~2.425）	0.343	-	-
术后放化疗（否）	1.354（1.142~2.308）	0.036	1.401（1.145~2.324）	0.046
ELK3表达（阳性）	2.266（1.606~3.196）	0.000	2.843（1.587~5.092）	0.000

影响因素	单因素		多因素
影响因素	HR（95%CI）值	P值	HR（95%CI）值	P值
性别（男）	0.739（0.523~1.051）	0.682	-	-
年龄（≥60岁）	0.284（0.061~1.206）	0.349	-	-
肿瘤大小（≥4 cm）	1.979（1.621~2.417）	0.008	1.133（0.433~2.948）	0.732
浸润深度（T3-T4）	1.852（1.004~3.414）	0.047	0.706（0.345~1.443）	0.340
淋巴结转移（有）	1.633（1.112~2.401）	0.000	2.219（1.223~4.026）	0.000
肿瘤分期（Ⅲ-Ⅳ期）	1.258（1.057~3.035）	0.034	1.067（1.021~3.143）	0.039
分化程度（低分化）	2.301（1.331~3.979）	0.003	1.466（1.074~2.201）	0.028
病理分型	0.898（0.735~1.104）	0.133	-	-
神经侵犯（是）	2.271（1.276~4.338）	0.007	1.273（1.143~3.086）	0.031
脉管侵犯（是）	1.413（0.823~2.425）	0.343	-	-
术后放化疗（否）	1.354（1.142~2.308）	0.036	1.401（1.145~2.324）	0.046
ELK3表达（阳性）	2.266（1.606~3.196）	0.000	2.843（1.587~5.092）	0.000

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