Abstract:
Objective To investigate the effect of siRNA targeted silencing of Livin gene on multidrug resistance of human breast cancer MCF-7 cells.
Methods ADM low concentration gradient induced method combined with high-dose intermittent was used to establish multidrug resistance cell line 5-FU induced by ATP in vitro, bioluminescence tumor drug detection (ATP-TCA) method was used for the detection of drug resistant cells, immunohistochemical method was used to detect the expression of Livin protein in MCF-7 resistant cell lines, using Livin combined with SiRNA epirubicin, fluorouracil, docetaxel , gemzar, apoptosis was induced in breast cancer MDR cells.
Results The results showed that there was a multidrug resistance problem in MCF-7 multidrug resistant cells, and the combination group MCF-7 was sensitized (19.48+ 12) and MCF-7/MDR was sensitized (35.62+ 2.37) mu M, and the data were statistically significant (P<0.05) compared with those in the transfected group and the addition group.
Conclusion siRNA specific targeted silencing of Livin gene can enhance the sensitivity of human breast cancer MCF-7 multidrug resistance cells and increase the rate of apoptosis.
Key words:
Breast Neoplasms,
RNA, Small Interfering,
Cell Line, Tumor,
Multidrug Resistance-Associated Proteins
Wenjin Duan, Shaoyou Li, Qian Yao, Aimei Jiang, Yun Ma. Study of siRNA specific targeted silencing of Livin gene on multidrug resistance in human breast cancer MCF-7 cells[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2018, 12(06): 507-509.