To investigate the pathogenic bacteria and drug resistance in patients’ bile after laparoscopic biliary tract surgery.

From October 2013 to September 2017, 560 patients received laparoscopic biliary tract surgery were enrolled into present study. Biliary bacteria were cultured and identified by API bacteria identification strip, then the agar diffusion method was used for drug susceptibility test, finally the WHONET 5.6 software (WHO bacterial resistance monitoring center) were used to analyze data rows.

Positive rate of bacterial culture in emergency surgery group (96.00%) was significantly higher than (5.00%) in elective surgery group (P<0.05); In all pathogenic bacteria, the account of Escherichia coli was the highest; Enterococcus faecalis to clindamycin and erythromycin resistance rate were more than 50%, Enterococcus faecalis to penicillin, ampicillin and levofloxacin, tetracycline, clindamycin, erythromycin, ciprofloxacin, gentamicin, streptomycin resistance rate were more than 50%; The coagulase negative staphylococci rate of resistant to penicillin, clindamycin and erythromycin were greater than 50%; rate of resistant Escherichia coli to cefuroxime, cefotaxime, ampicillin, sulfamethoxazole was greater than 50%, rate of Klebsiella pneumoniae resistant to cefepime, cefuroxime, cefotaxime, ampicillin, sulfamethoxazole was more than 50%. And the rate of proteus resistance to cefuroxime, cefotaxime, ampicillin, sulfamethoxazole and sulfamethoxazole was more than 50%.

Patients should not be treated with antibiotics if they could receive elective laparoscopic biliary surgery, and the drug sensitivity analysis should be done before they used antibiotics.