The 3D fluorescence reverse staining technique is contrary to the traditional ICG "positive staining" (tumor visualization). The reverse staining method involves targeted injection of indocyanine green (ICG) through the peripheral vein, which makes healthy liver tissue visible, while the tumor area shows "negative staining" due to the interruption of the portal vein branches and the lack of normal liver cells and biliary excretion function, thus precisely defining the boundaries of liver segments. Moreover, this technique has undergone three major innovations: precise visualization of anatomical boundaries, real-time intraoperative navigation, and ultra-minimally invasive operations, and has become a key support for radical liver cancer surgery. The team has independently developed an ultra-minimally invasive instrument that can be assembled and disassembled outside the body, solving the current problem that the small clamp heads of minimally invasive instruments less than 2 mm cannot meet clinical needs. Moreover, it leaves only a 2mm incision on the skin after the operation, and the postoperative cosmetic effect is consistent with that of conventional single-port laparoscopy, but it significantly reduces the operation time and operational difficulty. In this operation, two SY19-1.98B type ultra-minimally invasive instruments developed by the team and T-shaped sutures were used. Except for the umbilical wound, the single wound was less than 2mm. This video shows the operation process of 3D fluorescence reverse staining ultra-minimally invasive laparoscopic anatomical resection of right liver cancer assisted by this instrument.