Abstract:
Objective The expression levels of microrNA-98-5p (mir-98-5p) and ALKB homologous protein 1 (ALKBH1) in hilar cholangiocarcinoma (HC) were analyzed, and the relationship between microrNA-98-5P and clinicopathological features and prognosis of patients was discussed.
Methods Cancer tissues and corresponding adjacent normal tissues (≥5 cm) of 96 patients with HC who underwent radical surgery from June 2011 to June 2017 were selected as the study objects. Real-time quantitative fluorescent PCR (qRT-PCR) was used to detect the mRNA expression levels of mir-98-5p and ALKBH1 in all tissues, and immunohistochemistry was used to detect the expression levels of ALKBH1 in tissues. Statistical analysis was performed using SPSS 25.0, the measurement data were expressed as (
), independent sample t test was performed, and χ2 test was performed for comparison among the count groups. Pearson correlation was used to analyze the relationship between mir-98-5p and ALKBH1 mRNA expression levels in HC tissues. Kaplan-Meier survival curve was used to analyze the relationship between mir-98-5p, ALKBH1 and 5-year overall survival of HC patients. P<0.05 was considered statistically significant.
Results The relative expression level of ALKBH1 mRNA and positive rate of ALKBH1 in HC tissues were higher than those in adjacent normal tissues (P < 0.05), while the relative expression level of mir-98-5p was lower in HC tissues (P < 0.05). mir-98-5p and ALKBH1 were correlated with lymph node metastasis and TNM stage in HC patients (P < 0.05). The results of Pearson correlation analysis showed that mir-98-5p was negatively correlated with ALKBH1 mRNA expression in HC tissues (P < 0.05). Kaplan-Meier survival analysis showed that the 5-year cumulative survival rate of mir-98-5p low expression group was 18.8%, which was lower than that of mir-98-5p high expression group (33.3%) (P < 0.05). The 5-year cumulative survival rate of ALKBH1-positive group was 14.0%, and that of AlkBH1-negative group was 43.6% (P < 0.05).
Conclusion Low expression of mir-98-5p and high expression of ALKBH1 were correlated with lymph node metastasis, TNM stage and prognosis of HC patients.
Key words:
Bile Duct Neoplasms,
microRNA-98-5p,
ALKB Homolog 1,
Clinicopathological Characteristics
Jisiguleng Wu, Wa Hasigao. Expression of mir-98-5p and ALKBH1 in hilar cholangiocarcinoma and their relationship with clinicopathological features[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(02): 184-187.