With the advancement of minimally invasive surgical techniques, natural orifice specimen extraction surgery (NOSES) has been increasingly applied in the treatment of colorectal cancer. The NOSES-Ⅰ procedure for rectal cancer enables specimen extraction through the anus while adhering to the principles of radical oncologic resection and tumor-free technique, thereby avoiding an auxiliary abdominal incision. This approach offers advantages including reduced surgical trauma, less postoperative pain, fewer incision-related complications, and improved cosmetic outcomes. The robotic surgical system provides high-definition three-dimensional visualization and a flexible, stable operative platform, facilitating precise total mesorectal excision (TME) and gastrointestinal reconstruction within the narrow pelvis. However, this procedure requires careful patient selection, strict adherence to aseptic and tumor-free principles, and reliable anastomotic safety. This video demonstrates a case of robot-assisted NOSES-I surgery for rectal cancer, highlighting the minimally invasive advantages of combining robotic technology with the NOSES concept.