To explore the key operative technical points and clinical value of robot-assisted radical resection for low rectal cancer. With reference to the surgical video, we fully demonstrated the core operative steps of robot-assisted radical resection for low rectal cancer, including abdominal exploration, dissection and ligation of the inferior mesenteric vessels, preservation of pelvic autonomic nerves, total mesorectal excision, mobilization of the low rectum, and gastrointestinal reconstruction. This procedure confers particular technical advantages in addressing clinical challenges such as a narrow pelvis and low-lying tumors.The robotic system delivers magnified three-dimensional visualization and flexible robotic wrists, which facilitate meticulous dissection within the confined pelvic cavity. Standardized total mesorectal excision can be achieved with preservation of pelvic autonomic nerves and secure surgical resection margins. The approach reduces the technical difficulty of pelvic-floor manipulation and facilitates low sphincter-preserving surgery.The robotic surgical platform enables safe and standardized surgical management of low rectal cancer, with distinct advantages for meticulous deep-pelvic dissection. Proficiency in operative workflows and technical skills is essential to guarantee surgical quality. This procedure is suitable for implementation and popularization in well-equipped medical centers.