An experimental study on the promotion of colorectal cancer invasion and migration by high glucose through HGF/c-met pathway

doi:10.3877/cma.j.issn.1674-3946.2024.01.007

Abstract

Abstract:

Objective

To investigate the effect of high glucose on invasion and migration of colorectal cancer（CRC）through hepatocyte growth factor（HGF）/c-met pathway.

Methods

Forty CRC patients from January 2022 to December 2022 were selected and divided into hyperglycemic group（blood glucose ≥6.1 mmol/L，n=22）and normal blood glucose group（blood glucose 3.96.1 mmol/L，n=18）according to fasting blood glucose. The clinicopathological features and high expression rates of HGF and c-met were compared between the two groups. HCT116 cells were divided into control group（n=5），high glucose group（n=5）and high glucose +sull274 group（n=5）. The proliferation，invasion，migration，HGF and c-met protein expression levels of the three groups were compared. SPSS 20.0 statistical software was used to process the data. The measurement data were expressed as（

x ¯

±s）. One-way ANOVA analysis of variance was used for multi-group comparison，and LSD-t test was used for pin-group comparison. The comparison of counting data between the two groups was performed using the χ² test. P < 0.05 was considered statistically significant.

Results

The proportion of CRC differentiation and lymph node metastasis in hyperglycemia group were higher than those in normal glycemia group（P < 0.05）. The high expression rates of HGF and c-met in hyperglycemia group were higher than those in normal blood glucose group（P < 0.05）. Compared with the control group，the proliferation activity，invasion，migration，HGF and c-met protein expression levels of HCT116 cells in high glucose group were increased，with statistical significance（P < 0.05）. Compared with the high sugar group，the above indexes in the high sugar +sull274 group were lower，and the difference was statistically significant （P < 0.05）.

Conclusion

High glucose can promote CRC invasion and migration by upregulating HGF/c-met pathway.

Key words: Colorectal Neoplasms, High Sugar, Hepatocyte Growth Factor, Neoplasm Invasiveness, Cell Migration Assays

Shengjun Zhang, Ajing Zhao, Shoubo Li, Xianghong Hao, Minli Liu. An experimental study on the promotion of colorectal cancer invasion and migration by high glucose through HGF/c-met pathway[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(01): 21-24.

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临床特征	例	高血糖组（n=22）	血糖正常组（n=18）	P值
年龄(岁)
<55	19	8	11	>0.05
≥55	21	14	7
性别
男	27	16	11	>0.05
女	13	6	7
分化程度
低分化	20	15	5	<0.05
中高分化	20	7	13
淋巴结转移
无	22	8	14	<0.05
有	18	14	4
Dukes分期
A+B	20	9	11	>0.05
C+D	20	13	7
HGF
高表达	23	17	6	<0.05
低表达	17	5	12
c-met
高表达	21	15	6	<0.05
低表达	19	7	12

临床特征	例	高血糖组（n=22）	血糖正常组（n=18）	P值
年龄(岁)
<55	19	8	11	>0.05
≥55	21	14	7
性别
男	27	16	11	>0.05
女	13	6	7
分化程度
低分化	20	15	5	<0.05
中高分化	20	7	13
淋巴结转移
无	22	8	14	<0.05
有	18	14	4
Dukes分期
A+B	20	9	11	>0.05
C+D	20	13	7
HGF
高表达	23	17	6	<0.05
低表达	17	5	12
c-met
高表达	21	15	6	<0.05
低表达	19	7	12

组别	例	HGF	c-met
对照组	5	0.9±0.1	0.9±0.1
高糖组	5	1.6±0.2^a	1.5±0.1^a
高糖+sull274组	5	1.0±0.1^b	1.2±0.1^b

组别	例	HGF	c-met
对照组	5	0.9±0.1	0.9±0.1
高糖组	5	1.6±0.2^a	1.5±0.1^a
高糖+sull274组	5	1.0±0.1^b	1.2±0.1^b

组别	例	增殖活力(OD值)	侵袭细胞数	迁移细胞数
对照组	5	0.8±0.1	35.4±10.0	39.8±14.7
高糖组	5	1.3±0.2^a	71.1±20.0^a	74.5±19.5^a
高糖+sull274组	5	1.0±0.2^b	55.1±11.1^b	59.1±15.0^b

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