探讨血清脂肪酸（FA）代谢物及维生素D（VD）水平与结直肠癌（CRC）发生的相关性。

回顾性分析2019年1月至2021年12月41例初次诊断为CRC患者和41例健康体检者的临床资料，分别设为CRC组和健康组。采用液相色谱-质谱检测法分析部分相关的短链脂肪酸和中长链脂肪酸；采用酶联免疫吸附法（ELISA)）检测血清中25-羟基维生素D[25-(OH)D]水平。数据应用软件SPSS 22.0处理，25-(OH)D水平等计量资料采用（）表示，行独立样本t检验；VD分布情况等计数资料采用[例(%)]表示，行χ²检验；Logistic回归模型分析脂肪酸代谢物水平中影响CRC发生的危险因素。P<0.05为差异有统计学意义。

在所测定的 20种脂肪酸中，与健康组相比，CRC组仅有2-羟基月桂酸酯、二十碳二酸酯、庚二酸和4-甲基壬酰基肉碱4种脂肪酸的表达差异有统计学意义（P<0.05）。通过单因素和多因素分析结果显示，FA代谢物中2-羟基月桂酸酯及4-甲基壬酰基肉碱水平为CRC发生的独立危险因素（P<0.05）。两组患者VD分布情况比较差异有统计学意义（P<0.05）；且CRC组患者血清25-(OH)D水平显著低于健康组（P<0.05）。CRC 患者血清25-(OH)D水平与分化程度、pTNM分期及淋巴结转移情况密切相关，且差异有统计学意义（P<0.05）。

血清中脂肪酸代谢物2-羟基月桂酸酯、4-甲基壬酰基肉碱及血清25-(OH)D水平与CRC的发生密切相关，特定FA代谢物及VD水平可能作为预测CRC发生的血清学标志物。

To investigate the relationship between serum fatty acid (FA) metabolites and vitamin D (VD) levels and colorectal cancer (CRC).

The clinical data of 41 patients with first diagnosis of CRC and 41 healthy subjects from January 2019 to December 2021 were retrospectively analyzed, which were divided into CRC group and healthy group, respectively. Some related short-chain fatty acids and medium-long chain fatty acids were analyzed by liquid chromatography-mass spectrometry. The level of 25-(OH)D in serum was detected by enzyme-linked immunosorbent assay (ELISA). Data were processed by SPSS 22.0. Measurement data such as 25-(OH)D level were represented by () and independent sample t test was performed. Statistical data such as VD distribution were represented by [n (%)] and χ² test was performed. Logistic regression model was used to analyze the risk factors of CRC in fatty acid metabolites. P<0.05 was considered statistically significant.

Among the 20 fatty acids tested, only 4 fatty acids (2-hydroxylaurate, eicosanediate, heptadecic acid and 4-methylnonylcarnitine) in CRC group had significant differences compared with the healthy group (P<0.05). The results of univariate and multivariate analysis showed that the levels of 2-hydroxylaurate and 4-methylnonylcarnitine in FA metabolites were independent risk factors for CRC (P<0.05). There was statistical significance in VD distribution between the two groups (P<0.05). Moreover, the serum 25-hydroxyvitamin D[25-(OH)D] level in CRC group was significantly lower than that in healthy group (P<0.05). The level of serum 25-(OH)D in CRC patients was closely correlated with the degree of differentiation, pTNM stage and lymph node metastasis, and the differences were statistically significant (P<0.05).

Serum fatty acid metabolites 2-hydroxylaurate, 4-methylnonylcarnitine and serum 25-(OH)D are closely related to the occurrence of CRC. Specific FA metabolites and VD levels may be used as serological markers to predict the occurrence of CRC.