切换至 "中华医学电子期刊资源库"

中华普外科手术学杂志(电子版) ›› 2026, Vol. 20 ›› Issue (01) : 91 -95. doi: 10.3877/cma.j.issn.1674-3946.2026.01.025

综述

吲哚菁绿在肝胆胰外科中的应用
徐竟益1, 孙凯1,2, 王爽1, 吴柳盛1, 梁悦华1, 杨宇帆1, 赵雨桐1, 杨雷1, 王文强1, 闫军1,()   
  1. 1102218 北京,清华大学北京清华长庚医院肝胆胰外科
    2100084 北京,清华大学生物医学工程学院
  • 收稿日期:2025-06-20 出版日期:2026-02-26
  • 通信作者: 闫军

The application of indocyanine green in hepatobiliary and pancreatic surgery

Jingyi Xu1, Kai Sun1,2, Shuang Wang1, Liusheng Wu1, Yuehua Liang1, Yufan Yang1, Yutong Zhao1, Lei Yang1, Wenqiang Wang1, Jun Yan1,()   

  1. 1Department of Hepatobiliary Pancreatic Surgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing 102218, China
    2School of Biomedical Engineering, Tsinghua University, Beijing 100084, China
  • Received:2025-06-20 Published:2026-02-26
  • Corresponding author: Jun Yan
引用本文:

徐竟益, 孙凯, 王爽, 吴柳盛, 梁悦华, 杨宇帆, 赵雨桐, 杨雷, 王文强, 闫军. 吲哚菁绿在肝胆胰外科中的应用[J/OL]. 中华普外科手术学杂志(电子版), 2026, 20(01): 91-95.

Jingyi Xu, Kai Sun, Shuang Wang, Liusheng Wu, Yuehua Liang, Yufan Yang, Yutong Zhao, Lei Yang, Wenqiang Wang, Jun Yan. The application of indocyanine green in hepatobiliary and pancreatic surgery[J/OL]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2026, 20(01): 91-95.

随着荧光成像技术的不断进步,ICG作为一种近红外荧光染料,在肝胆胰外科领域展现出广泛应用前景。本文对ICG在该领域的应用进行了全面梳理和深度探讨。ICG凭借其独特的荧光特性,在肝功能评估方面,实现了对肝脏摄取和排泄功能的实时动态监测,为临床医生提供了更准确的肝脏功能状态信息;在肝脏肿瘤诊断与治疗中,ICG荧光成像技术以其高灵敏度和特异性,清晰地显示肿瘤边界和微小病灶,有力地支持了精准手术切除;在胆道应用方面,ICG使胆道造影更加清晰,有效降低了胆道损伤风险,并在胆瘘监测中发挥独特优势,助力及时发现和处理术后并发症;在胰腺应用方面,ICG为胰腺肿瘤的诊断和手术导航开辟了新的途径。然而,ICG在应用中也存在特异性有限、荧光信号易受干扰、成本较高等问题。

With the continuous advancement of fluorescence imaging technology, Indocyanine Green (ICG), as a near-infrared fluorescent dye, has demonstrated broad application prospects in the field of hepatobiliary and pancreatic surgery. This article conducts a comprehensive collation and in-depth exploration of the applications of ICG in this field. Leveraging its unique fluorescent properties, ICG achieves real-time dynamic monitoring of liver uptake and excretion functions in terms of liver function assessment, providing clinicians with more accurate information on the functional status of the liver. In the diagnosis and treatment of liver tumors, ICG fluorescence imaging technology, with its high sensitivity and specificity, clearly displays tumor boundaries and tiny lesions, strongly supporting precise surgical resection. In biliary applications, ICG enhances the clarity of cholangiography, effectively reducing the risk of biliary tract injury, and plays a unique role in bile leakage monitoring, facilitating the timely detection and management of postoperative complications. In pancreatic applications, ICG has opened up new avenues for the diagnosis of pancreatic tumors and surgical navigation.

[1]
Fransvea P, Chiarello MM, Fico V, et al. Indocyanine green: The guide to safer and more effective surgery[J]. World J Gastrointest Surg, 2024, 16(3): 641-649.
[2]
Miele D, Sorrenti M, Catenacci L, et al. Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles for Photodynamic Therapy [J]. Pharmaceutics, 2022, 14(8): 1740.
[3]
Hu Y, Wen T, Tuo B. The role of ICG NIRL fluorescence imaging in the surgical treatment of digestive system tumors (Review) [J]. Mol Med Rep, 2025, 32(1): 181.
[4]
Alotaibi H, Hatahet T, Al-Jamal WT. Indocyanine green J-aggregate (IJA) theranostics: Challenges and opportunities [J]. Int J Pharm, 2024, 661: 124456.
[5]
Ge Y, O’Shea DF. Review of Clinically Assessed Molecular Fluorophores for Intraoperative Image Guided Surgery[J]. Molecules, 2024, 29(24): 5964.
[6]
Zhao X, Li S, Song Y, et al. Construction of a near infrared fluorescence system for imaging of biological tissues [J]. Sci Rep, 2024, 14(1): 1626.
[7]
Takemura N, Ito K, Inagaki F, et al. Added value of indocyanine green fluorescence imaging in liver surgery [J]. Hepatobiliary Pancreat Dis Int, 2022, 21(4): 310-317.
[8]
Imamura H, Sano K, Sugawara Y, et al. Assessment of hepatic reserve for indication of hepatic resection: decision tree incorporating indocyanine green test [J]. J Hepatobiliary Pancreat Surg, 2005, 12(1): 16-22.
[9]
Piccolo G, Barabino M, Ghilardi G, et al. Detection and Real-Time Surgical Assessment of Colorectal Liver Metastases Using Near-Infrared Fluorescence Imaging during Laparoscopic and Robotic-Assisted Resections [J]. Cancers (Basel), 2024, 16(9): 1641.
[10]
Ishizawa T, Masuda K, Urano Y, et al. Mechanistic background and clinical applications of indocyanine green fluorescence imaging of hepatocellular carcinoma [J]. Ann Surg Oncol, 2014, 21(2): 440-448.
[11]
Kose E, Kahramangil B, Aydin H, et al. A comparison of indocyanine green fluorescence and laparoscopic ultrasound for detection of liver tumors [J]. HPB (Oxford), 2020, 22(5): 764-769.
[12]
刘克文, 庞志东. 吲哚菁绿荧光显影在复杂困难肝段肝细胞癌肝切除术的应用研究[J/CD]. 中华普外科手术学杂志(电子版), 2023, 17(03): 319-322.
[13]
Aoki T, Yasuda D, Shimizu Y, et al. Image-guided liver mapping using fluorescence navigation system with indocyanine green for anatomical hepatic resection[J]. World J Surg, 2008, 32(8): 1763-1767.
[14]
Ishizawa T, Zuker NB, Kokudo N, et al. Positive and negative staining of hepatic segments by use of fluorescent imaging techniques during laparoscopic hepatectomy[J]. Arch Surg, 2012, 147(4): 393-394.
[15]
唐梅, 周丽, 牛岑月, 等. ICG荧光导航的腹腔镜肝切除术临床意义[J/CD]. 中华普外科手术学杂志(电子版), 2024, 18(06): 655-658.
[16]
Berardi G, Igarashi K, Li CJ, et al. Parenchymal Sparing Anatomical Liver Resections With Full Laparoscopic Approach: Description of Technique and Short-term Results [J]. Ann Surg, 2021, 273(4): 785-791.
[17]
Wakabayashi T, Cacciaguerra AB, Abe Y, et al. Indocyanine Green Fluorescence Navigation in Liver Surgery: A Systematic Review on Dose and Timing of Administration [J]. Ann Surg, 2022, 275(6): 1025-1034.
[18]
刘青光, 宋涛, 王欢欢. 肝癌外科手术切除技术的再认识[J]. 中华消化外科杂志, 2024, 23(01): 75-79.
[19]
Liu L, Ma G, Zhang C, et al. An Activatable Theranostic Nanomedicine Platform Based on Self-Quenchable Indocyanine Green-Encapsulated Polymeric Micelles [J]. J Biomed Nanotechnol, 2016, 12(6): 1223-1233.
[20]
林伟鸿, 李凯. 肝切除术前肝脏储备功能评估方式的新进展[J]. 中华外科杂志, 2021, 59(05): 392-396.
[21]
魏林, 朱志军, 李宏宇, 等. 76例全腹腔镜活体肝移植供肝获取的回顾性分析[J]. 中华器官移植杂志, 2020, 41(11): 655-660.
[22]
张彤. 吲哚菁绿荧光成像在肝移植中的应用[J]. 中华肝胆外科杂志, 2019, 25(07): 543-545.
[23]
Ishizawa T, Bandai Y, Ijichi M, et al. Fluorescent cholangiography illuminating the biliary tree during laparoscopic cholecystectomy [J]. Br J Surg, 2010, 97(9): 1369-1377.
[24]
Ishizawa T, Bandai Y, Kokudo N. Fluorescent cholangiography using indocyanine green for laparoscopic cholecystectomy: an initial experience [J]. Arch Surg, 2009, 144(4): 381-382.
[25]
Matsumura M, Kawaguchi Y, Kobayashi Y, et al. Indocyanine green administration a day before surgery may increase bile duct detectability on fluorescence cholangiography during laparoscopic cholecystectomy [J]. J Hepatobiliary Pancreat Sci, 2021, 28(2): 202-210.
[26]
Pujol-Cano N, Molina-Romero FX, Palma-Zamora E, et al. Near-infrared fluorescence cholangiography at a very low dose of indocyanine green: quantification of fluorescence intensity using a colour analysis software based on the RGB color model[J]. Langenbecks Arch Surg, 2022, 407(8): 3513-3524.
[27]
黄振驹, 胡浩宇, 曾思略, 等. 三维可视化联合吲哚菁绿分子荧光影像技术在复杂性肝胆管结石诊疗中应用研究[J]. 中国实用外科杂志, 2023, 43(01): 108-113.
[28]
孙浩楠, 刘付宝. 荧光腹腔镜在肝胆胰外科的应用及实践[J]. 肝胆外科杂志, 2023, 31(05): 327-330.
[29]
刘文博, 张建业, 保积武, 等. 吲哚菁绿介导的近红外光检测技术在胆囊癌手术切除术中的应用[J]. 实用肝脏病杂志, 2019, 22(04): 573-576.
[30]
Ahmad A. Use of indocyanine green (ICG) augmented near-infrared fluorescence imaging in robotic radical resection of gallbladder adenocarcinomas [J]. Surg Endosc, 2020, 34(6): 2490-2494.
[31]
Yang J, Tao HS, Luo W, et al. A novel method of fluorescent imaging can guide hepatectomy for intrahepatic cholangiocarcinoma with intrahepatic biliary obstruction[J]. J Surg Oncol, 2020, 122(8): 1580-1586.
[32]
Zhang Y, Zhang Y, Zhu J, et al. Clinical application of indocyanine green fluorescence imaging in laparoscopic lymph node dissection for intrahepatic cholangiocarcinoma: A pilot study (with video) [J]. Surgery, 2022, 171(6): 1589-1595.
[33]
Kalata S, Thumma JR, Norton EC, et al. Comparative Safety of Robotic-Assisted vs Laparoscopic Cholecystectomy[J]. JAMA Surg, 2023, 158(12): 1303-1310.
[34]
Aoki T, Murakami M, Yasuda D, et al. Intraoperative fluorescent imaging using indocyanine green for liver mapping and cholangiography[J]. J Hepatobiliary Pancreat Sci, 2010, 17(5): 590-594.
[35]
Osayi SN, Wendling MR, Drosdeck JM, et al. Near-infrared fluorescent cholangiography facilitates identification of biliary anatomy during laparoscopic cholecystectomy[J]. Surg Endosc, 2015, 29(2): 368-375.
[36]
Dip F, LoMenzo E, Sarotto L, et al. Randomized Trial of Near-infrared Incisionless Fluorescent Cholangiography[J]. Ann Surg, 2019, 270(6): 992-999.
[37]
Xue S, Wang H, Chen X, et al. Risk factors of postoperative bile leakage after liver resection: A systematic review and meta-analysis [J]. Cancer Med, 2023, 12(14): 14922-14936.
[38]
Skipenko OG, Bedzhanyan AL, Chardarov NK, et al. Evaluation of the white test effectiveness for the prevention of bile leakage after liver resection: multicenter randomized controlled study [J]. Updates Surg, 2025. Online ahead of print.
[39]
Sakaguchi T, Suzuki A, Unno N, et al. Bile leak test by indocyanine green fluorescence images after hepatectomy[J]. Am J Surg, 2010, 200(1): e19-e23.
[40]
Kaibori M, Ishizaki M, Matsui K, et al. Intraoperative indocyanine green fluorescent imaging for prevention of bile leakage after hepatic resection [J]. Surgery, 2011, 50(1): 91-98.
[41]
Zhu M, Sheng Z, Jia Y, et al. Indocyanine Green-holo-Transferrin Nanoassemblies for Tumor-Targeted Dual-Modal Imaging and Photothermal Therapy of Glioma [J]. ACS Appl Mater Interfaces, 2017, 9(45): 39249-39258.
[42]
奚士航, 王小明, 王冠男, 等. 吲哚菁绿荧光成像在腹腔镜胰腺癌手术中的应用分析[J]. 中华肝胆外科杂志, 2024, 30(03): 193-196.
[43]
Qi B, Crawford AJ, Wojtynek NE, et al. Indocyanine green loaded hyaluronan-derived nanoparticles for fluorescence-enhanced surgical imaging of pancreatic cancer [J]. Nanomedicine, 2018, 14(3): 769-780.
[44]
朱倩, 杨智勇, 袁玉峰. 吲哚菁绿荧光显像技术在胰腺外科的应用[J]. 腹部外科, 2021, 34(04): 257-260, 270.
[45]
Newton AD, Predina JD, Shin MH, et al. Intraoperative Near-infrared Imaging Can Identify Neoplasms and Aid in Real-time Margin Assessment During Pancreatic Resection [J]. Ann Surg, 2019, 270(1): 12-20.
[1] 宋成顺, 吕小飞, 薛军, 赵舸. 吲哚菁绿荧光成像技术在胃肠肿瘤手术中的应用进展[J/OL]. 中华普通外科学文献(电子版), 2025, 19(03): 188-192.
[2] 艾世超, 孙艺文, 宋鹏, 沈晓菲, 刘颂, 孙锋, 陆晓峰, 王萌, 管文贤. 静脉注射吲哚菁绿导航胃癌根治术的单臂开放前瞻性研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(02): 166-169.
[3] 肖建, 肖天保, 陈江, 杨桃, 何峰, 保甜甜, 曹一波, 杨琴, 赵颖. 吲哚菁绿成像技术在保留左结肠动脉的直肠癌根治术中的应用价值[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(02): 134-137.
[4] 周世振, 朱兴亚, 袁庆港, 刘理想, 王凯, 缪骥, 丁超, 汪灏, 管文贤. 吲哚菁绿荧光成像技术在腹腔镜直肠癌侧方淋巴结清扫中的应用效果分析[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 44-47.
[5] 希龙夫, 薛荣泉. 人工智能在肝胆胰肿瘤诊治中应用与进展[J/OL]. 中华腔镜外科杂志(电子版), 2025, 18(03): 166-171.
[6] 李燕, 何丽, 侯倩男, 黄璐, 张强, 龚照林, 林永红. vNOTES 腹膜后前哨淋巴结活检在早期子宫内膜癌中的应用[J/OL]. 中华腔镜外科杂志(电子版), 2025, 18(01): 48-54.
[7] 吴哲境, 李敬东. ICG荧光成像引导下腹腔镜肝切除术治疗肝癌的安全性和有效性Meta分析[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(06): 852-859.
[8] 王浩然, 刘心远, 樊华. ICG荧光成像技术在移植肝灌注及功能评估中应用[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(06): 912-918.
[9] 卜俊峰, 陈炜. ICG在肝切除术中的应用进展[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(05): 780-784.
[10] 廖艳, 成伟. 近红外荧光成像技术在胰腺癌中的应用[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(05): 693-699.
[11] 王楚斯, 刘家伟, 卢逸, 汤照峰. ICG荧光显影在腹腔镜肝癌切除术中临床应用[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(04): 549-553.
[12] 甘翌翔, 欧阳俐颖, 潘扬勋, 张耀军, 陈敏山, 徐立. ICGR15和ALBI评分对肝动脉灌注化疗后肝癌肝切除术后肝衰竭和预后的预测价值[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(03): 395-401.
[13] 中华预防医学会肝胆胰疾病预防与控制专业委员会, 中国医疗保健国际交流促进会肝脏肿瘤学分会. 精准制导技术应用于肝脏肿瘤诊断与治疗中国专家共识(2024 版)[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(02): 145-157.
[14] 吴哲境, 李敬东, 熊永福, 刘刚, 陈雷. 吲哚菁绿在解剖性肝切除术中的应用[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(01): 25-29.
[15] 吴汉然, 柳常青, 吴显宁, 吴杲, 杜丽, 张莉, 梅新宇. 吲哚菁绿荧光腔镜胸导管显影术在预防微创McKeown食管癌切除术后乳糜胸中的疗效分析[J/OL]. 中华胸部外科电子杂志, 2025, 12(02): 55-65.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?